Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) ELISA Kit | NOX4 elisa kit

Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) ELISA Kit

Cat. Num. AAA2019064

• Gene Names: Nox4; AI648021
• Reactivity: Rat
• Synonyms: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4); Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) ELISA Kit; KOX; KOX-1; RENOX; NADPH Oxidase 4; NOX4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4).; No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) and analogues was observed.
• Sequence Length: 543

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NOX4 elisa kit

Signal transduction; Enzyme & Kinase; Metabolic pathway; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 552953832
• NCBI GeneID: 50490
• NCBI Accession #: NP_001272762.1
• NCBI GenBank Nucleotide #: NM_001285833.1
• UniProt Accession #: Q9JHI8; Q3TF39; Q8C3M1; Q8VCA3
• Molecular Weight: 71,244 Da
• NCBI Official Full Name: NADPH oxidase 4 isoform 2
• NCBI Official Synonym Full Names: NADPH oxidase 4
• NCBI Official Symbol: Nox4
• NCBI Official Synonym Symbols: AI648021
• NCBI Protein Information: NADPH oxidase 4
• UniProt Protein Name: NADPH oxidase 4
• Protein Family: NADPH oxidase
• UniProt Gene Name: Nox4
• UniProt Synonym Gene Names: Renox; KOX-1
• UniProt Entry Name: NOX4_MOUSE

Uniprot Description

NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 1.6.3.-; Nucleolus; Membrane protein, multi-pass; Membrane protein, integral; Oxidoreductase

Cellular Component: focal adhesion; membrane; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; stress fiber; cell junction; NADPH oxidase complex

Molecular Function: NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; oxidoreductase activity, acting on NADH or NADPH, with oxygen as acceptor; oxidoreductase activity

Biological Process: negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of MAP kinase activity; positive regulation of apoptosis; positive regulation of stress fiber formation; cell morphogenesis; superoxide metabolic process; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; superoxide release; cardiac muscle cell differentiation; bone resorption