Goat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit | NOX4 elisa kit

Goat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit

Cat. Num. AAA739286

• Gene Names: NOX4; KOX; KOX-1; RENOX
• Reactivity: Goat
• Synonyms: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4; Goat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit; NOX4 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This NOX4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat NOX4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX4 elisa kit

Principle of the Assay: NOX4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NOX4 antibody and an NOX4 -HRP conjugate. The assay sample and buffer are incubated together with NOX4 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX4 concentration since NOX4 from samples and NOX4 -HRP conjugate compete for the anti-NOX4 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX4 from the sample, fewer sites are left to bind NOX4 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NOX4 elisa kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 219842348
• NCBI GeneID: 50507
• NCBI Accession #: NP_001137309.1
• NCBI GenBank Nucleotide #: NM_001143837.1
• UniProt Accession #: Q9NPH5; Q5K3R4; Q5K3R5; Q5K3R6; Q5K3R8; Q7Z7G3; Q86V92; A8K715
• Molecular Weight: 66,932 Da
• NCBI Official Full Name: NADPH oxidase 4 isoform c
• NCBI Official Synonym Full Names: NADPH oxidase 4
• NCBI Official Symbol: NOX4
• NCBI Official Synonym Symbols: KOX; KOX-1; RENOX
• NCBI Protein Information: NADPH oxidase 4; kidney oxidase-1; renal NAD(P)H-oxidase; kidney superoxide-producing NADPH oxidase
• UniProt Protein Name: NADPH oxidase 4
• Protein Family: NADPH oxidase
• UniProt Gene Name: NOX4
• UniProt Synonym Gene Names: RENOX; KOX-1
• UniProt Entry Name: NOX4_HUMAN

NCBI Description

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Uniprot Description

NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.6.3.-; Cell cycle regulation; Membrane protein, multi-pass; Nucleolus

Chromosomal Location of Human Ortholog: 11q14.2-q21

Cellular Component: endoplasmic reticulum membrane; focal adhesion; mitochondrion; perinuclear region of cytoplasm; apical plasma membrane; nucleolus; stress fiber; integral to membrane; NADPH oxidase complex

Molecular Function: oxygen sensor activity; electron carrier activity; FAD binding; NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; heme binding; nucleotide binding

Biological Process: positive regulation of protein kinase B signaling cascade; negative regulation of cell proliferation; positive regulation of MAP kinase activity; positive regulation of apoptosis; positive regulation of stress fiber formation; cell morphogenesis; response to hypoxia; cell aging; homocysteine metabolic process; inflammatory response; positive regulation of smooth muscle cell migration; superoxide release; cardiac muscle cell differentiation; bone resorption