Nitric Oxide Synthase 3, Endothelial (NOS3) Recombinant Protein | NOS3 recombinant protein

Recombinant Nitric Oxide Synthase 3, Endothelial (NOS3)

Cat. Num. AAA2011673

• Gene Names: NOS3; cNOS; eNOS; EC-NOS; NOSIII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Nitric Oxide Synthase 3; Endothelial (NOS3); Recombinant Nitric Oxide Synthase 3; NOS3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-ASILYASET VRAQSYAQQL GRLFRKAFDP RVLCMDEYDV VSLEHETLVL VVTSTFGNGD PPENGESFAA ALMEMSGPYN GSPRPEQHRS YKIRFNSVSC SDPLVSSWRR KRKESSNTDS AGALGTLRFC VFGLGSRAYP HFCAFARAVD TRLEELGGER LLQLGQGDEL CGQEEAFRGW AQAAF
• Sequence Length: 1205

• Applicable Applications for NOS3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Ala522~Phe705 (Accession # Q28969) with N-terminal His-Tag
• Subcellular Location: Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Cell membrane
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47523660
• NCBI GeneID: 397557
• NCBI Accession #: NP_999460.1
• NCBI GenBank Nucleotide #: NM_214295.1
• UniProt Accession #: Q28969
• Molecular Weight: 21.9kDa
• NCBI Official Full Name: nitric oxide synthase, endothelial
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: cNOS; eNOS; EC-NOS; NOSIII
• NCBI Protein Information: nitric oxide synthase, endothelial; NOS type III; endothelial NOS; constitutive NOS
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: NOS; cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_PIG

Uniprot Description

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN

By similarity.

Subunit structure: Homodimer. Interacts with NOSIP and NOSTRIN

By similarity.

Subcellular location: Membrane › caveola

By similarity. Cytoplasm › cytoskeleton

By similarity. Golgi apparatus

By similarity. Cell membrane

By similarity. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity

By similarity.

Induction: Repressed by proinflammatory cytokines.

Post-translational modification: Phosphorylation by AMPK at Ser-1179 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-497, resulting in inhibition of activity. Phosphorylation of Ser-116 by CDK5 reduces activity

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.