Nitric Oxide Synthase 1, Neuronal (NOS1) Recombinant Protein | NOS1 recombinant protein

Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)

Cat. Num. AAA2012434

• Gene Names: Nos1; NO; bNOS; nNOS; NOS-I; Nos-1; 2310005C01Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
• Purity: >92%
• Synonyms: Nitric Oxide Synthase 1; Neuronal (NOS1); Recombinant Nitric Oxide Synthase 1; NOS1 recombinant protein

• Host: E Coli
• Purity/Purification: >92%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-FHQEMLNYRL TPSFEYQPDP WNTHVWKGTN GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA KRVKATILYA TETGKSQAYA KTLCEIFKHA FDAKAMSMEE YDIVHLEHEA LVLVVTSTFG NGDPPENGEK FGCALM
• Sequence Length: 1429

• Applicable Applications for NOS1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Phe691~Met826 with N-terminal His-Tag
• Tissue Specificity: Brain.
• Subcellular Location: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine.
• Traits: Freeze-dried powder
• Predicted isoelectric point: 7.9
• Predicted Molecular Mass: 16.8kDa
• Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6724321
• NCBI GeneID: 18125
• NCBI Accession #: NP_032738.1
• NCBI GenBank Nucleotide #: NM_008712.2
• UniProt Accession #: Q9Z0J4; Q3UR10; Q64208
• NCBI Official Full Name: nitric oxide synthase, brain
• NCBI Official Synonym Full Names: nitric oxide synthase 1, neuronal
• NCBI Official Symbol: Nos1
• NCBI Official Synonym Symbols: NO; bNOS; nNOS; NOS-I; Nos-1; 2310005C01Rik
• NCBI Protein Information: nitric oxide synthase, brain; N-NOS; NC-NOS; NOS type I; neuronal NOS; constitutive NOS; neuronal nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS1
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_MOUSE

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Cellular Component: sarcoplasmic reticulum membrane; nuclear membrane; protein complex; photoreceptor inner segment; mitochondrion; sarcoplasmic reticulum; postsynaptic density; T-tubule; dendrite; caveola; vesicle membrane; cytosol; Z disc; lipid raft; azurophil granule; mitochondrial outer membrane; cell projection; cytoskeleton; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; nucleus; sarcolemma

Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; metal ion binding; oxidoreductase activity; cadmium ion binding; ATPase binding; calmodulin binding; protein binding; enzyme binding; sodium channel regulator activity; NADPH-hemoprotein reductase activity; FMN binding; iron ion binding; heme binding; NADP binding

Biological Process: negative regulation of serotonin uptake; response to peptide hormone stimulus; striated muscle contraction; negative regulation of heart contraction; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; negative regulation of peptidyl-serine phosphorylation; positive regulation of vasodilation; regulation of heart contraction; negative regulation of insulin secretion; negative regulation of cell proliferation; behavioral response to cocaine; negative regulation of hydrolase activity; negative regulation of blood pressure; nitric oxide biosynthetic process; negative regulation of calcium ion transport; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; negative regulation of vasoconstriction; response to estrogen stimulus; response to heat; reduction of cytosolic calcium ion concentration; exogenous drug catabolic process; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; regulation of sensory perception of pain; nitric oxide mediated signal transduction; multicellular organismal response to stress; negative regulation of apoptosis