VWC2 recombinant protein

VWC2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121071

• Gene Names: VWC2; UNQ739; PSST739
• Purity: >95% as determined by SDS-PAGE
• Synonyms: VWC2; VWC2 Protein; Human; Recombinant (His Tag); Human VWC2/Brorin Protein (His Tag); PSST739 Protein; UNQ739 Protein; von Willebrand factor C domain containing 2; VWC2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met325

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human VWC2 (NP_940972.2)(Met 1-Met 325) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for VWC2 recombinant protein

Background: Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein that contains two VWFC domains. VWC2/Brorin is a BMP antagonist that may play a role in neural development. It promotes cell adhesion. VWC2/Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2/Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2/Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.

References

Koike,N. et al., 2007, J Biol Chem. 282 (21):15843-50. Elis,S. et al., 2009,Mol Reprod Dev. 76 (11):1043-55. Zhang,J.L. et al., 2010,PLoS One. 5 (9):e12846.

NCBI and Uniprot Product Information

• NCBI GI #: 260654089
• NCBI GeneID: 375567
• NCBI Accession #: NP_940972.2
• NCBI GenBank Nucleotide #: NM_198570.3
• UniProt Accession #: Q2TAL6; Q6UXE2
• Molecular Weight: 35,282 Da
• NCBI Official Full Name: brorin
• NCBI Official Synonym Full Names: von Willebrand factor C domain containing 2
• NCBI Official Symbol: VWC2
• NCBI Official Synonym Symbols: UNQ739; PSST739
• NCBI Protein Information: brorin; brain-specific chordin-like protein; von Willebrand factor C domain-containing protein 2
• UniProt Protein Name: Brorin
• Protein Family: Brorin
• UniProt Gene Name: VWC2
• UniProt Entry Name: VWC2_HUMAN

Uniprot Description

PSST739: BMP antagonist which may play a role in neural development. Promotes cell adhesion.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: extracellular space; basement membrane; synapse; cell junction

Biological Process: positive regulation of neuron differentiation; negative regulation of BMP signaling pathway