Bovine Neurokinin B ELISA Kit | NKB elisa kit

Bovine Neurokinin B ELISA Kit

Cat. Num. AAA753153

• Gene Names: TAC3; NKB; HH10; NKNB; PRO1155; ZNEUROK1
• Reactivity: Bovine
• Synonyms: Neurokinin B; Bovine Neurokinin B ELISA Kit; NKB elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NKB elisa kit

Principle of the assay: NKB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NKB antibody and an NKB-HRP conjugate. The assay sample and buffer are incubated together with NKB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NKB concentration since NKB from samples and NKB-HRP conjugate compete for the anti-NKB antibody binding site. Since the number of sites is limited, as more sites are occupied by NKB from the sample, fewer sites are left to bind NKB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NKB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NKB elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 8572053
• NCBI GeneID: 6866
• Molecular Weight: 11,358 Da
• NCBI Official Full Name: neurokinin B
• NCBI Official Synonym Full Names: tachykinin 3
• NCBI Official Symbol: TAC3
• NCBI Official Synonym Symbols: NKB; HH10; NKNB; PRO1155; ZNEUROK1
• NCBI Protein Information: tachykinin-3; neurokinin b; neuromedin K; gamma tachykinin 3; preprotachykinin B; preprotachykinin-B
• UniProt Protein Name: Tachykinin-3
• UniProt Gene Name: TAC3
• UniProt Synonym Gene Names: NKNB; UNQ585/PRO1155; NKB
• UniProt Entry Name: TKNK_HUMAN

NCBI Description

This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded protein is primarily expressed in the central and peripheral nervous system and functions as a neurotransmitter. This protein is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; receptor binding

Biological Process: tachykinin signaling pathway; neuropeptide signaling pathway; female pregnancy

Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia