NGF recombinant protein

Recombinant Mouse NGF Protein

Cat. Num. AAA8249497

• Gene Names: Ngf; Ngfb
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: NGF; Recombinant Mouse NGF Protein; NGFB; Beta-nerve growth factor; Beta-NGF; NGF recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Sequence: SSTHPVFHMG EFSVCDSVSV WVGDKTTATD IKGKEVTVLA EVNINNSVFR QYFFETKCRA SNPVESGCRG IDSKHWNSYC TTTHTFVKAL TTDEKQAAWR FIRIDTACVC VLSRKATRRG
• Sequence Length: 241

• Applicable Applications for NGF recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for NGF recombinant protein

Recombinant Mouse NGF Protein is produced by our E Coli expression system and the target gene encoding Ser122-Gly241 is expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 162951831
• NCBI GeneID: 18049
• NCBI Accession #: NP_001106168.1
• NCBI GenBank Nucleotide #: NM_001112698.2
• UniProt Accession #: P01139; Q63864; Q6LDB7
• Molecular Weight: 27,077 Da
• NCBI Official Full Name: beta-nerve growth factor isoform B
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF
• UniProt Entry Name: NGF_MOUSE

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cytoplasmic vesicle; extracellular region; extracellular space

Molecular Function: growth factor activity; metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; receptor signaling protein activity; transmembrane receptor protein tyrosine kinase activator activity

Biological Process: adult locomotory behavior; circadian rhythm; induction of apoptosis via death domain receptors; memory; negative regulation of neuron apoptosis; neurite development; neurite morphogenesis; neuron apoptosis; peripheral nervous system development; positive regulation of axon extension; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of collateral sprouting; positive regulation of nerve growth factor receptor signaling pathway; positive regulation of neuron differentiation; positive regulation of neuron maturation; positive regulation of protein amino acid autophosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of protein ubiquitination; positive regulation of Ras protein signal transduction; positive regulation of transcription factor activity; regulation of neuron differentiation; regulation of neurotransmitter secretion; regulation of release of sequestered calcium ion into cytosol; sensory perception of pain