beta Nerve Growth Factor Recombinant Protein | NGF recombinant protein

Human beta Nerve Growth Factor Recombinant

Cat. Num. AAA553200

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: beta Nerve Growth Factor; Human beta Nerve Growth Factor Recombinant; ngf-b; nerve growth factor; beta ngf; NGF recombinant protein

• Host: Chinese Hamster Ovary Cells (CHO)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: NGF-beta was lyophilized from a 0.2 um filtered solution in 20mM sodium acetate, 150mM NaCl, pH 5.5.
• Sequence: MSMLFYTLIT AFLIGIQAEP HSESNVPAGH TIPQAHWTKL QHSLDTALRR ARSAPAAAIA ARVAGQTRNI TVDPRLFKKR RLRSPRVLFS TQPPREAADT QDLDFEVGGA APFNRTHRSK RSSSHPIFHR GEFSVCDSVS VWVGDKTTAT DIKGKEVMVL GEVNINNSVF KQYFFETKCR DPNPVDSGCR GIDSKHWNSY CTTTHTFVKA LTMDGKQAAW RFIRIDTACV CVLSRKAVRR A
• Sequence Length: 241

• Domain: NGF
• Host Note: Optimized DNA sequence encoding Human Nerve Growth Factor mature chain was expressed in CHO cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human Nerve Growth Factor is generated by the proteolytic removal of the signal peptide and propeptide the molecule has a calculated molecular mass of approximately 27 kDa.; ; Recombinant human NGF is as a non disulfide-linked homodimeric protein consisting of two 118 amino acid residue subunits, and migrates as a 13 - 14 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for NGF recombinant protein

NGF is mainly responsible for the survival and the differentiation and the functional activities of sensory and sympathetic neurons in the peripheral nervous system. It also plays an important role in the development and functional activities of cholinergic neurons in the central nervous system. Since NGF is synthesized also in non-neuronal tissues it may have a much wider spectrum of biological activities than thought previously. NGF stimulates chemotactic migration of human polymorphonuclear leukocytes in vitro. NGF stimulates the growth and differentiation of B- cells and the growth of T- cells and of some tumor cell types. NGF inhibits immunoglobulin production by various human plasma cell. The cytokines IL1, IL6, and bFGF are potent inducers of NGF. NGF induces the synthesis of IL1 in pheochromocytoma cells which in turn acts as a growth factor for glial cells and induces the synthesis of NGF following nerve injuries.

Product Categories/Family for NGF recombinant protein

Cytokines

References

• in vitro evidence of Nerve Growth Factor effects on human conjunctival epithelial cell differentiation and mucin gene expression .Invest. Ophthalmol. Vis. Sci., Oct 2009; 50: 4622 - 4630.
• identification of critical residues within the conserved and specificity patches of Nerve Growth Factor leading to survival or differentiation .J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.058420.
• neuroactive conducting scaffolds: Nerve Growth Factor conjugation on active ester-functionalized polypyrrole .J R Soc Interface, Sep 2009; 6: 801 - 810.
• Nerve Growth Factor enhances cough and airway obstruction via trka receptor- and trpv1-dependent mechanisms .Thorax, Sep 2009; 64: 791 - 797.
• erk5 activity is required for Nerve Growth Factor-induced neurite outgrowth and stabilization of tyrosine hydroxylase in pc12 cells .J. Biol. Chem., Aug 2009; 284: 23564 - 23573.