NFu1 recombinant protein

NFu1, 10-254aa, Human, His tag, E Coli

Cat. Num. AAA205994

• Gene Names: NFU1; Nfu; NifU; HIRIP; MMDS1; NIFUC; CGI-33; HIRIP5
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: NFu1; 10-254aa; Human; His tag; E Coli; NFu1 iron-sulfur cluster scaffold homolog mitochondrial; NFu1 iron-sulfur cluster scaffold homolog; mitochondrial; CGI-33; HIRIP; HIRIP5; MMDS1; Nfu; Nifu; NIFuC; NFu1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffer saline (pH 7.4) containing 20% glycerol 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSGAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPV RYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENE ELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKG FEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP
• Sequence Length: 254

• Applicable Applications for NFu1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NFu1 recombinant protein

NFu1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFu1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

References

Ganesh S., et al (2003). Hum. Mol. Genet. 12:2359-2368; Tong W.-H., et al (2003). Proc. Natl. Acad. Sci. u.S.A. 100:9762-9767;

NCBI and Uniprot Product Information

• NCBI GI #: 50593021
• NCBI GeneID: 27247
• NCBI Accession #: NP_001002755
• NCBI GenBank Nucleotide #: NM_001002755.2
• UniProt Accession #: Q9UMS0; Q53QE5; Q6VNZ8; Q7Z5B1; Q7Z5B2; Q9Y322; B4DUL9
• Molecular Weight: 29.9 kDa (268aa) confirmed by MALDI-TOF
• NCBI Official Full Name: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial isoform 2
• NCBI Official Synonym Full Names: NFU1 iron-sulfur cluster scaffold
• NCBI Official Symbol: NFU1
• NCBI Official Synonym Symbols: Nfu; NifU; HIRIP; MMDS1; NIFUC; CGI-33; HIRIP5
• NCBI Protein Information: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
• UniProt Protein Name: NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
• Protein Family: NifU-like protein
• UniProt Gene Name: NFU1

NCBI Description

This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Iron-sulfur cluster scaffold protein which can assemble [4Fe-2S] clusters and deliver them to target proteins.