Inhibitory Subunit Of NF Kappa B Zeta (IkBz) Recombinant Protein | IkBz recombinant protein

Recombinant Inhibitory Subunit Of NF Kappa B Zeta (IkBz)

Cat. Num. AAA2018344

• Gene Names: Nfkbiz; INAP; Mail; AA408868
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Inhibitory Subunit Of NF Kappa B Zeta (IkBz); Recombinant Inhibitory Subunit Of NF Kappa B Zeta (IkBz); IkBz recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MGSPMNT TQLGKSFFQW QVEQEESKLA NIPQDQFLAR DGDGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG QSAFQVAVAA NQHLIVQDLV NLGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVRSN QFVDLEATNY DGLTPLHCAV VAHNAVVHEL QRNRQSHSPE VQDLLLRNKS LVDTIKCLIQMGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS FVNA
• Sequence Length: 728

• Applicable Applications for IkBz recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met414~Ala654 (Accession # Q9EST8) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 226874841
• NCBI GeneID: 80859
• NCBI Accession #: NP_001152866.1
• NCBI GenBank Nucleotide #: NM_001159394.1
• UniProt Accession #: Q9EST8; Q3TVL7; Q3TWK9; Q3U8Y9; Q3UCI2; Q3URP0; Q3UW99; Q5NT97; Q99NA4
• Molecular Weight: 30.4kDa
• NCBI Official Full Name: NF-kappa-B inhibitor zeta isoform a
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta
• NCBI Official Symbol: Nfkbiz
• NCBI Official Synonym Symbols: INAP; Mail; AA408868
• NCBI Protein Information: NF-kappa-B inhibitor zeta; ikB-zeta; ikappaBzeta; IkappaB-zeta; I-kappa-B-zeta; IL-1 inducible nuclear ankyrin-repeat protein; molecule possessing ankyrin repeats induced by lipopolysaccharide; molecule possessing ankyrin-repeats induced by lipopolysaccharide; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• UniProt Protein Name: NF-kappa-B inhibitor zeta
• UniProt Gene Name: Nfkbiz
• UniProt Synonym Gene Names: Inap; Mail; IkB-zeta; IkappaBzeta; INAP; MAIL
• UniProt Entry Name: IKBZ_MOUSE

Uniprot Description

IkB-zeta: Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF- kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis. Interacts with NFKB1/p50. Interacts with RELA. By TNF, IL1/interleukin-1 and bacterial lipopolysaccharides (LPS). Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: nucleus

Molecular Function: transcription cofactor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; inflammatory response