NEu1 recombinant protein

NEu1, 48-415aa, Human, His tag, E Coli

Cat. Num. AAA204313

• Gene Names: NEU1; NEU; NANH; SIAL1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: NEu1; 48-415aa; Human; His tag; E Coli; Sialidase 1 (lysosomal sialidase); NANH; NEu; SIAL1; NEu1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: GSSHHHHHH SSGLVPRGSH MGSHMENDFG LVQPLVTMEQ LLWVSGRQIG SVDTFRIPLI TATPRGTLLA FAEARKMSSS DEGAKFIALR RSMDQGSTWS PTAFIVNDGD VPDGLNLGAV VSDVETGVVF LFYSLCAHKA GCQVASTMLV WSKDDGVSWS TPRNLSLDIG TEVFAPGPGS GIQKQREPRK GRLIVCGHGT LERDGVFCLL SDDHGASWRY GSGVSGIPYG QPKQENDFNP DECQPYELPD GSVVINARNQ NNYHCHCRIV LRSYDACDTL RPRDVTFDPE LVDPVVAAGA VVTSSGIVFF SNPAHPEFRV NLTLRWSFSN GTSWRKETVQ LWPGPSGYSS LATLEGSMDG EEQAPQLYVL YEKGRNHYTE SISVAKISVY GTL
• Sequence Length: 48-415aa

• Applicable Applications for NEu1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for NEu1 recombinant protein

NEu1 is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red -myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. Recombinant human NEu1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NEu1 recombinant protein

Metabolism

References

Abdulkhalek,S., et al. (2011) J. Biol. Chem. 286 (42), 36532-36549

NCBI and Uniprot Product Information

• NCBI GI #: 4557791
• NCBI GeneID: 4758
• NCBI Accession #: NP_000425
• NCBI GenBank Nucleotide #: NM_000434.3
• UniProt Accession #: Q99519
• Molecular Weight: 42.9 kDa (393aa), confirmed by MALDI-TOF
• NCBI Official Full Name: sialidase-1
• NCBI Official Synonym Full Names: neuraminidase 1
• NCBI Official Symbol: NEU1
• NCBI Official Synonym Symbols: NEU; NANH; SIAL1
• NCBI Protein Information: sialidase-1
• UniProt Protein Name: Sialidase-1
• Protein Family: Sialidase
• UniProt Gene Name: NEU1
• UniProt Synonym Gene Names: NANH

NCBI Description

The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.