Nei Endonuclease VIII-Like 1 Recombinant Protein | NEIL1 recombinant protein

Recombinant Human Nei Endonuclease VIII-Like 1

Cat. Num. AAA145391

• Gene Names: NEIL1; FPG1; NEI1; hFPG1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Nei Endonuclease VIII-Like 1; Recombinant Human Nei Endonuclease VIII-Like 1; NEIL1 Human; Nei Endonuclease VIII-Like 1 Human Recombinant; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1; NEIL1; NEI1; hFPG1; NEIL1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The NEIL1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS.
• Sequence Length: 476

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for NEIL1 recombinant protein

Description: NEIL1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 410 amino acids (1-390) and having a molecular mass of 45.8kDa.NEIL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: NEIL1 is a member of a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. NEIL1 participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines.

Product Categories/Family for NEIL1 recombinant protein

Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 375065857
• NCBI GeneID: 79661
• NCBI Accession #: NP_001243481.1
• NCBI GenBank Nucleotide #: NM_001256552.1
• UniProt Accession #: Q96FI4; Q6ZRA7; Q86XW7; Q9H6C3; D3DW75
• Molecular Weight: 43,684 Da
• NCBI Official Full Name: endonuclease 8-like 1 isoform 1
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 1 (E. coli)
• NCBI Official Symbol: NEIL1
• NCBI Official Synonym Symbols: FPG1; NEI1; hFPG1
• NCBI Protein Information: endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; NEH1; endonuclease VIII; endonuclease VIII-like 1; nei homolog 1; nei-like protein 1
• UniProt Protein Name: Endonuclease 8-like 1
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL1
• UniProt Synonym Gene Names: NEH1
• UniProt Entry Name: NEIL1_HUMAN

NCBI Description

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.

Protein type: Lyase; EC 4.2.99.18; DNA repair, damage; Hydrolase

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; DNA N-glycosylase activity; hydrolase activity, acting on glycosyl bonds; damaged DNA binding

Biological Process: nucleotide-excision repair; base-excision repair; response to oxidative stress; DNA catabolic process, endonucleolytic; negative regulation of nuclease activity