NCF2 recombinant protein

Recombinant Human NCF2 protein

Cat. Num. AAA1562201

• Gene Names: NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NCF2; Recombinant Human NCF2 protein; Neutrophil cytosol factor 2; NCF-2; 67kDa neutrophil oxidase factor; NADPH oxidase activator 2; Neutrophil NADPH oxidase factor 2; p67-phox; NCF2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human NCF2 (Lys355-Val526) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for NCF2 recombinant protein

NCF2, also known as NCF-2 and p67phox, is a subunit of the multi-protein NADPH oxidase complex. NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.

Product Categories/Family for NCF2 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 1346669
• NCBI GeneID: 4688
• UniProt Accession #: P19878; Q2PP06; Q8NFC7; Q9BV51; B2R6Q1; B4DKQ7; B4DQA7; E9PHJ2; E9PHX3
• Molecular Weight: 59,762 Da
• NCBI Official Full Name: Neutrophil cytosol factor 2
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 2
• NCBI Official Symbol: NCF2
• NCBI Official Synonym Symbols: NCF-2; NOXA2; P67PHOX; P67-PHOX
• NCBI Protein Information: neutrophil cytosol factor 2; NADPH oxidase activator 2; 67 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 2; chronic granulomatous disease, autosomal 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
• UniProt Protein Name: Neutrophil cytosol factor 2
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF2
• UniProt Synonym Gene Names: NOXA2; P67PHOX; NCF-2
• UniProt Entry Name: NCF2_HUMAN

NCBI Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytoplasm; nucleolus; acrosome; cytosol; NADPH oxidase complex

Molecular Function: protein C-terminus binding; protein binding; electron carrier activity; Rac GTPase binding

Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to lipopolysaccharide; cellular response to hormone stimulus; antigen processing and presentation of peptide antigen via MHC class I; response to hyperoxia; positive regulation of neuron apoptosis; response to glucose stimulus; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; response to activity; response to progesterone stimulus; vascular endothelial growth factor receptor signaling pathway; superoxide release; positive regulation of blood pressure; aging

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii