FIBRONECTIN Native Protein | FN1 native protein
PURIFIED BOVINE FIBRONECTIN
Gene Names
FN1; FN
Applications
ELISA
Purity
>/=95% by SDS PAGE
Synonyms
FIBRONECTIN; PURIFIED BOVINE FIBRONECTIN; FN1 native protein
Purity/Purification
>/=95% by SDS PAGE
Form/Format
Purified
Purified bovine fibronectin - sterile liquid
Purified bovine fibronectin - sterile liquid
Concentration
Protein concentration 0.5 mg/ml (varies by lot)
Sequence Length
2478
Applicable Applications for FN1 native protein
ELISA (EIA), Tissue Culture (TC)
Application Notes
Tissue Culture: Minimum Dilution: 1; Maximum Dilution: 10 ug/ml
Instructions For Use
Preparation of Fibronectin:
Generally fibronectin should be stored frozen or refrigerated at 1-5 mg/ml. If frozen, thaw at 37 degree C. Fibronectin should never be vortexed or treated roughly as it precipitates out of solution easily (visible as a granulated precipitate or as a slimy strand or clumps). Any observed insoluble aggregates are very difficult to resuspend. If precipitation is observed, the solution should initially be warmed to 37 degree C with gentle shaking, followed by aseptic filtration via a 5 um (or smaller) cut off syringe filter.
It is also recommended that fibronectin should not be stored long term in buffer solutions containing Mg2+ or Ca2+ as these can contribute to fibronectin precipitation over time.
General Coating Procedure (for 96-well plates):
1. Plates: Choice of plates can affect the amount of protein successfully coated. MyBioSource recommends the use of commercially available high protein binding plates.
2. Add between 1-10 ug/ml of fibronectin in PBS buffer for an overnight (or longer) incubation at 2-8 degree C (100 ul/well). The optimum amount of protein to saturate the plate surface should be determined by the user. For ELISA applications some users have coated with carbonate buffer pH 9.0 but this is generally not necessary and can damage the cell binding activity of the fibronectin.
3. Regardless of assay type (cell binding, ELISA etc.), it is necessary to block the remaining protein adherence sites on the plate. Therefore, in a separate step add a blocking protein such as 2-5% BSA in PBS for at least 1 hr at room temperature or overnight at 2-8 degree C (200 ul/well). Caution: The BSA solution should be 0.2 um filtered prior to use to remove excess non-specific binding effects in the assay caused by insoluble BSA clumps. The use of RIA-grade (radio-immunoassay grade, suitable for immunoassays) BSA is recommended.
4. Blocked plates can stored in the refrigerator for several weeks or can be decanted and dried and stored for months in a dessicator. Dessicated material should be rehydrated for 15 min with PBS before use.
Generally fibronectin should be stored frozen or refrigerated at 1-5 mg/ml. If frozen, thaw at 37 degree C. Fibronectin should never be vortexed or treated roughly as it precipitates out of solution easily (visible as a granulated precipitate or as a slimy strand or clumps). Any observed insoluble aggregates are very difficult to resuspend. If precipitation is observed, the solution should initially be warmed to 37 degree C with gentle shaking, followed by aseptic filtration via a 5 um (or smaller) cut off syringe filter.
It is also recommended that fibronectin should not be stored long term in buffer solutions containing Mg2+ or Ca2+ as these can contribute to fibronectin precipitation over time.
General Coating Procedure (for 96-well plates):
1. Plates: Choice of plates can affect the amount of protein successfully coated. MyBioSource recommends the use of commercially available high protein binding plates.
2. Add between 1-10 ug/ml of fibronectin in PBS buffer for an overnight (or longer) incubation at 2-8 degree C (100 ul/well). The optimum amount of protein to saturate the plate surface should be determined by the user. For ELISA applications some users have coated with carbonate buffer pH 9.0 but this is generally not necessary and can damage the cell binding activity of the fibronectin.
3. Regardless of assay type (cell binding, ELISA etc.), it is necessary to block the remaining protein adherence sites on the plate. Therefore, in a separate step add a blocking protein such as 2-5% BSA in PBS for at least 1 hr at room temperature or overnight at 2-8 degree C (200 ul/well). Caution: The BSA solution should be 0.2 um filtered prior to use to remove excess non-specific binding effects in the assay caused by insoluble BSA clumps. The use of RIA-grade (radio-immunoassay grade, suitable for immunoassays) BSA is recommended.
4. Blocked plates can stored in the refrigerator for several weeks or can be decanted and dried and stored for months in a dessicator. Dessicated material should be rehydrated for 15 min with PBS before use.
Buffer Solution
Phosphate buffered saline
Target Species
Bovine
Preparation and Storage
Store at 4 degree C or at -20 degree C for long term storage. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for FN1 native protein
Fibronectin is a 450kDa glycoprotein involved in cell adhesion and migration processes including embryogenesis, metastasis, wound healing, blood coagulation and host immunity.Fibronectin may be used as a coating on tissue culture surfaces to promote attachment, spreading and proliferation of various cell types.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
272,154 Da
NCBI Official Full Name
Fibronectin
NCBI Official Synonym Full Names
fibronectin 1<
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN
NCBI Protein Information
fibronectin; cumulus cell-specific fibronectin 1 transcript; embryo-specific fibronectin 1 transcript; fibronectin ED-A- region; fibronectin variable region
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN
UniProt Entry Name
FINC_BOVIN
Uniprot Description
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts ().
Research Articles on FN1
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Product Notes
The FN1 fn1 (Catalog #AAA230381) is a Native Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FIBRONECTIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Tissue Culture (TC). Tissue Culture: Minimum Dilution: 1; Maximum Dilution: 10 ug/ml. Researchers should empirically determine the suitability of the FN1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FIBRONECTIN, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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