Nuclear Autoantigenic Sperm Protein Recombinant Protein | NASP recombinant protein

Recombinant Nuclear Autoantigenic Sperm Protein, Histone Binding (NASP)

Cat. Num. AAA2032935

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Nuclear Autoantigenic Sperm Protein; Recombinant Nuclear Autoantigenic Sperm Protein; Histone Binding (NASP); NASP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: TESTATAA IAAELVSADK VEDAPAPSTS ADKMESLDVD SEAKKLLGLG QKHLVMGDIP AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR MENGVLGNAL EGVHVEEEEG EKTEDESLVE NNDNVDEEAR EELREQVYDA MGEKEAKKAE GQSLTKPETD KEQESEVEKGGREDMDISEP AEKLQEKVES TSKQLTESSE EAKEAAIPGL NED
• Sequence Length: 776

• Applicable Applications for NASP recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Thr3~Asp223
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 53850614
• NCBI GeneID: 298441
• NCBI Accession #: NP_001005543.1
• NCBI GenBank Nucleotide #: NM_001005543.1
• Molecular Weight: 84,200 Da
• NCBI Official Full Name: nuclear autoantigenic sperm protein
• NCBI Official Synonym Full Names: nuclear autoantigenic sperm protein (histone-binding)
• NCBI Official Symbol: Nasp
• NCBI Protein Information: nuclear autoantigenic sperm protein
• UniProt Protein Name: Nuclear autoantigenic sperm protein
• Protein Family: Nuclear autoantigenic sperm protein
• UniProt Gene Name: Nasp
• UniProt Synonym Gene Names: NASP
• UniProt Entry Name: NASP_RAT

Uniprot Description

NASP: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. Belongs to the NASP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; protein complex

Molecular Function: histone binding; Hsp90 protein binding; protein complex binding

Biological Process: blastocyst development; cell proliferation; DNA replication; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; histone exchange; male gonad development; nucleosome assembly; protein transport; response to testosterone stimulus