Rabbit anti-Human KARS Polyclonal Antibody | anti-KARS antibody

KARS, NT (KARS, KIAA0070, Lysine-tRNA ligase, Lysyl-tRNA synthetase) (MaxLight 650)

Cat. Num. AAA6312090

• Gene Names: KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: KARS; Polyclonal Antibody; NT (KARS; KIAA0070; Lysine-tRNA ligase; Lysyl-tRNA synthetase) (MaxLight 650); Lysine--tRNA ligase; Lysyl-tRNA synthetase; anti-KARS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-KARS antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KARS Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the KARS antibody detected the KARS protein (arrow).)

Western Blot (WB)

(Western blot analysis of KARS (arrow) using rabbit polyclonal KARS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.)

Flow Cytometry (FC/FACS)

(KARS Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KARS antibody

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-KARS antibody

Antibodies; Enzymes

References

McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010). Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010). Segat, L., et al. Vaccine 28(10):2201-2206(2010). Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010). Kawamata, H., et al. J. Biol. Chem. 283(42):28321-28328(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5031815
• NCBI GeneID: 3735
• NCBI Accession #: NP_005539.1; NP_001123561.1
• NCBI GenBank Nucleotide #: NM_005548.2
• UniProt Accession #: Q15046; O14946; Q96J25; Q9HB23; A8MSK1; D3DUK4
• Molecular Weight: 71,497 Da
• NCBI Official Full Name: lysine--tRNA ligase isoform 2
• NCBI Official Synonym Full Names: lysyl-tRNA synthetase
• NCBI Official Symbol: KARS
• NCBI Official Synonym Symbols: KRS; KARS1; KARS2; CMTRIB; DFNB89
• NCBI Protein Information: lysine--tRNA ligase; lysRS; lysine tRNA ligase
• UniProt Protein Name: Lysine--tRNA ligase
• UniProt Gene Name: KARS
• UniProt Synonym Gene Names: KIAA0070; LysRS
• UniProt Entry Name: SYK_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.1.1.6; Translation; Ligase; Mitochondrial

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: microtubule cytoskeleton; aminoacyl-tRNA synthetase multienzyme complex; mitochondrion; mitochondrial matrix; cytoplasm; extracellular region; plasma membrane; nucleus; cytosol

Molecular Function: amino acid binding; protein binding; metal ion binding; lysine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; lysyl-tRNA aminoacylation; viral reproduction; tRNA processing; gene expression; diadenosine tetraphosphate biosynthetic process

Disease: Deafness, Autosomal Recessive 89; Charcot-marie-tooth Disease, Recessive Intermediate B