Myosin VA Recombinant Protein | MYO5A recombinant protein

Recombinant Myosin VA (MYO5A)

Cat. Num. AAA2029755

• Gene Names: MYO5A; GS1; MYO5; MYH12; MYR12
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Myosin VA; Recombinant Myosin VA (MYO5A); MYO5A recombinant protein

• Host: Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Concentration: 8.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SLLTSTINSI KKVLKKRGDD FETVSFWLSN TCRFLHCLKQ YSGEEGFMKH NTSRQNEHCL TNFDLAEYRQ VLSDLAIQIY QQLVRVLENI LQPMIVSGML EHETIQGVSG VKPTGLRKRT SSIADEGTYT LDSILRQLNS FHSVMCQHGM DPELIKQVVK QMFYIIGAIT LNNLLLRKDM CSWSKGMQIR YNVSQLEEWL RDKNLMNSGA KETLEPLIQA AQLLQVKKKT DDDAEAICSM CNALTTAQIV KVLNLYTPVN EFEERVSVSF IRTIQMRLRD RKDSPQLLMD AKHIFPVTFP FNPSSLALET IQIPASLGLGFISRV
• Sequence Length: 128

• Applicable Applications for MYO5A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted isoelectric point: 8.6
• Predicted Molecular Mass: 40.9kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ser1531~Val1855
• Usage: Reconstitute in sterile ddH2O.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 3064064
• NCBI GeneID: 4644
• NCBI Official Full Name: myosin Va, partial
• NCBI Official Synonym Full Names: myosin VA (heavy chain 12, myoxin)
• NCBI Official Symbol: MYO5A
• NCBI Official Synonym Symbols: GS1; MYO5; MYH12; MYR12
• NCBI Protein Information: unconventional myosin-Va
• UniProt Protein Name: Unconventional myosin-Va
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO5A
• UniProt Synonym Gene Names: MYH12
• UniProt Entry Name: MYO5A_HUMAN

NCBI Description

This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

Uniprot Description

MYO5A: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. May also be required for some polarization process involved in dendrite formation. May be a homodimer, which associates with multiple calmodulin or myosin light chains. Binds MLPH and MYRIP. Interacts with RIPL2, the interaction is required for its role in dendrite formation. Detected in melanocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: Golgi apparatus; filopodium tip; neuron projection; lysosome; endoplasmic reticulum; early endosome; intermediate filament; actomyosin; actin filament; cytosol; ruffle; recycling endosome; growth cone; photoreceptor outer segment; cell soma; membrane; late endosome; cytoplasm; melanosome; peroxisome; myosin complex; vesicle

Molecular Function: calmodulin binding; microfilament motor activity; calcium ion binding; actin binding; Rab GTPase binding; ATP binding

Biological Process: myelination; exocytosis; ER localization; actin filament-based movement; long-chain fatty acid biosynthetic process; melanosome transport; locomotion during locomotory behavior; melanin biosynthetic process; odontogenesis; vesicle-mediated transport; vesicle transport along actin filament; synaptic transmission; cellular protein metabolic process; visual perception; cellular response to insulin stimulus; insulin secretion; transport; melanocyte differentiation; synapse organization and biogenesis; regulation of inositol-1,4,5-triphosphate receptor activity; post-Golgi vesicle-mediated transport; secretory granule localization

Disease: Griscelli Syndrome, Type 1