SDS-Page
Myosin IB (MYO1B) Recombinant Protein | MYO1B recombinant protein
Recombinant Myosin IB (MYO1B)
Gene Names
Myo1b; AA406997; myosin-1b
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Myosin IB (MYO1B); Recombinant Myosin IB (MYO1B); MYO1B recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-CK EAATTIAAYW HGTQARRELK RLKEEARRKH AVAVIWAYWL GLKVRREYRK FFRANAGKKI YEFTLQRIVQ KYLLEMKNKM PSLSPIDKNW PSRPYLFLDS THKELKRIFH LWRCKKYRDQ FTDQQKLIYE EKLEASELFK DKKALYPSSV GQPFQGAYLE INKNPKYKKL KDAIEEKIII AEVVNKINRA NGKSTSRIFL LTNNNLLLAD QKSGQIKSEV PLVDVTKVSM SSQNDGFFAV HLKEGSEAAS KGDFLFSSDH
MGHHHHHHSGS-CK EAATTIAAYW HGTQARRELK RLKEEARRKH AVAVIWAYWL GLKVRREYRK FFRANAGKKI YEFTLQRIVQ KYLLEMKNKM PSLSPIDKNW PSRPYLFLDS THKELKRIFH LWRCKKYRDQ FTDQQKLIYE EKLEASELFK DKKALYPSSV GQPFQGAYLE INKNPKYKKL KDAIEEKIII AEVVNKINRA NGKSTSRIFL LTNNNLLLAD QKSGQIKSEV PLVDVTKVSM SSQNDGFFAV HLKEGSEAAS KGDFLFSSDH
Sequence Length
1107
Applicable Applications for MYO1B recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Cys779~His1040 (Accession # P46735) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31.8kDa
NCBI Official Full Name
unconventional myosin-Ib isoform 2
NCBI Official Synonym Full Names
myosin IB
NCBI Official Symbol
Myo1b
NCBI Official Synonym Symbols
AA406997; myosin-1b
NCBI Protein Information
unconventional myosin-Ib; MMIa; MIH-L; MMI-alpha; myosin I alpha; molecular motor; Myosin IA (Myosin I alpha) (MMI-alpha) (MMIa) (MIH-L)
UniProt Protein Name
Unconventional myosin-Ib
Protein Family
UniProt Gene Name
Myo1b
UniProt Synonym Gene Names
MMI-alpha; MMIa
UniProt Entry Name
MYO1B_MOUSE
Uniprot Description
MYO1B: Motor protein that may participate in process critical to neuronal development and function such as cell migration, neurite outgrowth and vesicular transport. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motor; Actin-binding; Motility/polarity/chemotaxis
Cellular Component: perinuclear region of cytoplasm; cytoplasm; early endosome; plasma membrane; trans-Golgi network membrane; endosome membrane; actin filament; myosin complex; brush border; filopodium
Molecular Function: microfilament motor activity; actin filament binding; calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; motor activity; nucleotide binding; actin-dependent ATPase activity; actin binding; ATP binding
Biological Process: actin filament bundle formation; metabolic process; actin filament-based movement; actin filament organization; post-Golgi vesicle-mediated transport
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Product Notes
The MYO1B myo1b (Catalog #AAA2009680) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Myosin IB (MYO1B) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the MYO1B myo1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG S-CK EAATTIAAYW HGTQARRELK RLKEEARRKH AVAVIWAYWL GLKVRREYRK FFRANAGKKI YEFTLQRIVQ KYLLEMKNKM PSLSPIDKNW PSRPYLFLDS THKELKRIFH LWRCKKYRDQ FTDQQKLIYE EKLEASELFK DKKALYPSSV GQPFQGAYLE INKNPKYKKL KDAIEEKIII AEVVNKINRA NGKSTSRIFL LTNNNLLLAD QKSGQIKSEV PLVDVTKVSM SSQNDGFFAV HLKEGSEAAS KGDFLFSSDH. It is sometimes possible for the material contained within the vial of "Myosin IB (MYO1B), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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