Rat Myosin light chain 3 ELISA Kit | Myl3 elisa kit

Rat Myosin light chain 3 ELISA Kit

Cat. Num. AAA288414

• Gene Names: Myl3; MLClV; Mylc1v; rVMLC1
• Reactivity: Rat
• Synonyms: Myosin light chain 3; Rat Myosin light chain 3 ELISA Kit; Myl3 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Myosin light chain 3. No significant cross-reactivity or interference was observed.
• Sequence Length: 195

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.089 ng/mL
• Intra-Assay CV: <=4.5%
• Inter-Assay CV: <=9.5%
• Recovery: 98%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Myl3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin light chain 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myosin light chain 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myosin light chain 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Myosin light chain 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981240
• NCBI GeneID: 24585
• NCBI Accession #: NP_036738.1
• NCBI GenBank Nucleotide #: NM_012606.2
• UniProt Accession #: P16409
• Molecular Weight: 22,156 Da
• NCBI Official Full Name: myosin light chain 3
• NCBI Official Synonym Full Names: myosin light chain 3
• NCBI Official Symbol: Myl3
• NCBI Official Synonym Symbols: MLClV; Mylc1v; rVMLC1
• NCBI Protein Information: myosin light chain 3
• UniProt Protein Name: Myosin light chain 3
• Protein Family: Myosin
• UniProt Gene Name: Myl3
• UniProt Synonym Gene Names: rVMLC1
• UniProt Entry Name: MYL3_RAT

NCBI Description

predominant myosin light chain in cardiac tissue [RGD, Feb 2006]

Uniprot Description

MYL3: Regulatory light chain of myosin. Does not bind calcium. Defects in MYL3 are the cause of familial hypertrophic cardiomyopathy type 8 (CMH8). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.

Protein type: Motor; Motility/polarity/chemotaxis

Cellular Component: A band; I band; myosin complex

Molecular Function: actin monomer binding; calcium ion binding; motor activity

Biological Process: cardiac muscle contraction; muscle contraction; regulation of striated muscle contraction; regulation of the force of heart contraction; skeletal muscle development; ventricular cardiac muscle morphogenesis