Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) Recombinant Protein | MYH6 recombinant protein

Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)

Cat. Num. AAA2011106

• Gene Names: Myh6; Myhca
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myosin Heavy Chain 6; Cardiac Muscle; Alpha (MYH6); Recombinant Myosin Heavy Chain 6; MYH6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-QV YYSIGALAKS VYEKMFNWMV TRINATLETK QPRQYFIGVL DIAGFEIFDF NSFEQLCINF TNEKLQQFFN HHMFVLEQEE YKKEGIEWEF IDFGMDLQAC IDLIEKPMGI MSILEEECMF PKATDMTFKA KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLEKNKDP LNETVVGLYQ KSSLKLMATL FSTYASADTG DSGKGKGGKK KGSSFQTVSA LHRENLNKLM TNLRTT
• Sequence Length: 1938

• Applicable Applications for MYH6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gln419~Thr666 (Accession # P02563) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 127741
• NCBI GeneID: 29556
• UniProt Accession #: P02563; Q63351
• Molecular Weight: 30.0kDa
• NCBI Official Full Name: Myosin-6
• NCBI Official Synonym Full Names: myosin, heavy chain 6, cardiac muscle, alpha
• NCBI Official Symbol: Myh6
• NCBI Official Synonym Symbols: Myhca
• NCBI Protein Information: myosin-6; myHC-alpha; myosin heavy chain 6; myosin heavy chain, cardiac muscle alpha isoform; myosin, heavy polypeptide 6, cardiac muscle, alpha; myosin heavy chain polypeptide 6 cardiac muscle adult; myosin heavy chain, polypeptide 6, cardiac muscle, alpha
• UniProt Protein Name: Myosin-6
• Protein Family: Myosin
• UniProt Gene Name: Myh6
• UniProt Synonym Gene Names: MyHC-alpha
• UniProt Entry Name: MYH6_RAT

NCBI Description

heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

Uniprot Description

MYH6: Muscle contraction. Defects in MYH6 are the cause of atrial septal defect type 3 (ASD3). ASD3 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Defects in MYH6 are the cause of familial hypertrophic cardiomyopathy type 14 (CMH14). It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations,and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH6 are the cause of cardiomyopathy dilated type 1EE (CMD1EE). It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH6 are the cause of susceptibility to sick sinus syndrome type 3 (SSS3). The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. MYH6 variations are associated with susceptibility to sick sinus syndrome (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987).

Protein type: Motility/polarity/chemotaxis; Motor

Cellular Component: nucleoplasm; focal adhesion; myofibril; mitochondrion; cytoplasm; stress fiber; muscle myosin complex; nucleus; myosin complex; Z disc

Molecular Function: calmodulin binding; microfilament motor activity; identical protein binding; protein homodimerization activity; protein heterodimerization activity; ATPase activity; actin binding; actin-dependent ATPase activity; protein kinase binding; ATP binding; calcium-dependent ATPase activity

Biological Process: adult heart development; striated muscle contraction; actin filament-based movement; regulation of heart rate; atrial cardiac muscle morphogenesis; metabolic process; in utero embryonic development; sarcomere organization; Wnt receptor signaling pathway through beta-catenin; regulation of heart contraction; visceral muscle development; muscle filament sliding; BMP signaling pathway; regulation of ATPase activity; myofibril assembly; muscle contraction; response to heat; cardiac muscle fiber development; regulation of blood pressure; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction