Thermus Aquaticus DNA Mismatch Repair Recombinant Protein | MutS recombinant protein
Recombinant Thermus Aquaticus DNA Mismatch Repair Protein MutS
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Thermus Aquaticus DNA Mismatch Repair; Recombinant Thermus Aquaticus DNA Mismatch Repair Protein MutS; MutS; Thermus Aquaticus DNA Mismatch Repair Protein; DNA mismatch repair protein MutS.; MutS recombinant protein
Host
E. coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
The MutS protein (1mg/ml) is supplied in 20mM Tris-HCl, pH-8, 250mM NaCl, 0.1mM EDTA, 1mM DTT and 50% Glycerol.
Sequence
MKIEHMEGML KGEGPGPLPP LLQQYVELRD QYPDYLLLFQ VGDFYECFGE DAERLARALG LVLTHKTSKD FTTPMAGIPL RAFEAYAERL LKMGFRLAVA DQVEPAEEAE GLVRREVTQL LTPGTLLQES LLPREANYLA AIATGDGWGL AFLDVSTGEF KGTVLKSKSA LYDELFRHRP AEVLLAPELL ENGAFLDEFR KRFPVMLSEA PFEPEGEGPL ALRRARGALL AYAQRTQGGA LSLQPFRFYD PGAFMRLPEA TLRALEVFEP LRGQDTLFSV LDETRTAPGR RLLQSWLRHP LLDRGPLEAR LDRVEGFVRE GALREGVRRL LYRLADLERL ATRLELGRAS PKDLGALRRS LQILPELRAL LGEEVGLPDL SPLKEELEAA LVEDPPLKVS EGGLIREGYD PDLDALRAAH REGVAYFLEL EERERERTGI PTLKVGYNAV FGYYLEVTRP YYERVPKEYR PVQTLKDRQR YTLPEMKEKE REVYRLEALI RRREEEVFLE VRERAKRQAE ALREAARILA ELDVYAALAE VAVRYGYVRP RFGDRLQIRA GRHPVVERRT EFVPNDLEMA HELVLITGPN MAGKSTFLRQ TALIALLAQV GSFVPAEEAH LPLFDGIYTR IGASDDLAGG KSTFMVEMEE VALILKEATE NSLVLLDEVG RGTSSLDGVA IATAVAEALH ERRAYTLFAT HYFELTALGL PRLKNLHVAA REEAGGLVFY HQVLPGPASK SYGVEVAAMA GLPKEVVARA RALLQAMAAR REGALDAVLE RLLALDPDRL TPLEALRLLQ ELKALALGAP LDTMKGKLAA ALEHHHHHH.
Sequence Length
811
Application Notes
Recommended Applications:
1. Remove mismatch DNA (error correction) from gene synthesis reaction
2. Mutation detection and removal
3. Rapid isothermal SNP detection
1. Remove mismatch DNA (error correction) from gene synthesis reaction
2. Mutation detection and removal
3. Rapid isothermal SNP detection
Physical Appearance
Sterile filtered colorless solution.
Reaction Buffer
100mM KCl, 50mM Tris-HCl, pH-8.5, 20mM MgCl2, 0.1mM EDTA, 1mM DTT and 2% Glycerol, 65°C.
Protocol
1. After first round PCR, purify PCR fragments using Qiagen QIAquick PCR purification kit with elution in dH2O.
2. Dilute PCR product to 250 ng/ul in 10 mM Tris–HCl, pH 7.8, 50 mM NaCl and heat to 95°C for 5 min followed by cooling at 0.1°C /s to 25°C.
3. Add binding buffer (20 mM Tris–HCl, pH 7.8, 10 mM NaCl, 5 mM MgCl2, 1 mM DTT and 5% glycerol) to annealed PCR product, adjust DNA concentration to 11.5 ng/ul, add MutS dimers to 950 nM.
4. Incubate the mixture at room temperature for 10 min, then add an equal volume of Ni-NTA beads pre-equilibrated with 1×binding buffer, and incubate for 30 min at room temperature.
5. Gently spin down beads and save supernatant for subsequent processing (second round PCR, cloning etc).
2. Dilute PCR product to 250 ng/ul in 10 mM Tris–HCl, pH 7.8, 50 mM NaCl and heat to 95°C for 5 min followed by cooling at 0.1°C /s to 25°C.
3. Add binding buffer (20 mM Tris–HCl, pH 7.8, 10 mM NaCl, 5 mM MgCl2, 1 mM DTT and 5% glycerol) to annealed PCR product, adjust DNA concentration to 11.5 ng/ul, add MutS dimers to 950 nM.
4. Incubate the mixture at room temperature for 10 min, then add an equal volume of Ni-NTA beads pre-equilibrated with 1×binding buffer, and incubate for 30 min at room temperature.
5. Gently spin down beads and save supernatant for subsequent processing (second round PCR, cloning etc).
Preparation and Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Related Product Information for MutS recombinant protein
DNA Mismatch Repair Protein MutS Thermus Aquaticus Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 829 amino acids and having a molecular mass of 92.8kDa. The Thermus Aquaticus is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Categories/Family for MutS recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
DNA mismatch repair protein MutS
UniProt Protein Name
DNA mismatch repair protein MutS
Protein Family
UniProt Gene Name
mutS
Uniprot Description
This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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Product Notes
The MutS muts (Catalog #AAA141325) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. Recommended Applications: 1. Remove mismatch DNA (error correction) from gene synthesis reaction 2. Mutation detection and removal 3. Rapid isothermal SNP detection. Researchers should empirically determine the suitability of the MutS muts for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKIEHMEGML KGEGPGPLPP LLQQYVELRD QYPDYLLLFQ VGDFYECFGE DAERLARALG LVLTHKTSKD FTTPMAGIPL RAFEAYAERL LKMGFRLAVA DQVEPAEEAE GLVRREVTQL LTPGTLLQES LLPREANYLA AIATGDGWGL AFLDVSTGEF KGTVLKSKSA LYDELFRHRP AEVLLAPELL ENGAFLDEFR KRFPVMLSEA PFEPEGEGPL ALRRARGALL AYAQRTQGGA LSLQPFRFYD PGAFMRLPEA TLRALEVFEP LRGQDTLFSV LDETRTAPGR RLLQSWLRHP LLDRGPLEAR LDRVEGFVRE GALREGVRRL LYRLADLERL ATRLELGRAS PKDLGALRRS LQILPELRAL LGEEVGLPDL SPLKEELEAA LVEDPPLKVS EGGLIREGYD PDLDALRAAH REGVAYFLEL EERERERTGI PTLKVGYNAV FGYYLEVTRP YYERVPKEYR PVQTLKDRQR YTLPEMKEKE REVYRLEALI RRREEEVFLE VRERAKRQAE ALREAARILA ELDVYAALAE VAVRYGYVRP RFGDRLQIRA GRHPVVERRT EFVPNDLEMA HELVLITGPN MAGKSTFLRQ TALIALLAQV GSFVPAEEAH LPLFDGIYTR IGASDDLAGG KSTFMVEMEE VALILKEATE NSLVLLDEVG RGTSSLDGVA IATAVAEALH ERRAYTLFAT HYFELTALGL PRLKNLHVAA REEAGGLVFY HQVLPGPASK SYGVEVAAMA GLPKEVVARA RALLQAMAAR REGALDAVLE RLLALDPDRL TPLEALRLLQ ELKALALGAP LDTMKGKLAA ALEHHHHHH. It is sometimes possible for the material contained within the vial of "Thermus Aquaticus DNA Mismatch Repair, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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