Rabbit TMP21 Polyclonal Antibody | anti-TMED10 antibody

TMP21 Antibody

Cat. Num. AAA151447

• Gene Names: TMED10; p23; TMP21; S31I125; Tmp-21-I; S31III125; P24(DELTA)
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: TMP21 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TMP21; Polyclonal Antibody; TMP21 Antibody; p23; S31I125; Tmp-21-I; S31III125; P24(DELTA); Transmembrane emp24 domain-containing protein 10; 21 kDa transmembrane-trafficking protein; transmembrane emp24-like trafficking protein 10 (yeast); anti-TMED10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TMP21 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-TMED10 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TMP21 antibody was raised against a 18 amino acid synthetic peptide from near the center of human TMP21.
• Buffer: TMP21 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TMP21 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TMP21 in mouse brain tissue lysate with TMP21 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of TMP21 in human brain tissue with TMP21 antibody at 5 μg/mL.)

Related Product Information for anti-TMED10 antibody

TMP21 Antibody: TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta; production, demonstrating that TMP21 can modulate gamma-secretase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 4885697
• NCBI GeneID: 10972
• UniProt Accession #: P49755; Q15602; Q16536; Q86TC2; Q86TS5; B2R605
• Molecular Weight: 24,976 Da
• NCBI Official Full Name: TMP21
• NCBI Official Synonym Full Names: transmembrane emp24-like trafficking protein 10 (yeast)
• NCBI Official Symbol: TMED10
• NCBI Official Synonym Symbols: p23; TMP21; S31I125; Tmp-21-I; S31III125; P24(DELTA)
• NCBI Protein Information: transmembrane emp24 domain-containing protein 10; p24delta; p24delta1; p24 family protein delta-1; transmembrane protein Tmp21; 21 kDa transmembrane trafficking protein; 21 kDa transmembrane-trafficking protein
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 10
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMED10
• UniProt Synonym Gene Names: TMP21; S31I125; p24delta1
• UniProt Entry Name: TMEDA_HUMAN

NCBI Description

This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8. [provided by RefSeq, Jul 2008]

Uniprot Description

TMED10: Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus. Predominantly homodimeric and to lesser extent monomeric in endoplasmic reticulum. Homodimer and monomer in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED9. Interacts with TMED2. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED10. Interacts with ARF1 (GDP- bound); the interaction probably involves a TMED10 oligomer. Interacts with SEC23A; indicative for an association of TMED10 with the COPII vesicle coat. Interacts with CD59, SEC24B, SEC24C and SEC24D. Interacts with MPPE1/PGAP5. Interacts with F2LR1. Interacts with KDELR2; the interaction is disrupted by KDELR2 ligand. Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin- dependent gamma-secretase complex. Ubiquitous. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; zymogen granule membrane; cis-Golgi network; secretory granule membrane; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane; Golgi membrane; COPI-coated vesicle; trans-Golgi network transport vesicle; melanosome; plasma membrane

Molecular Function: protein binding; syntaxin binding; protein complex binding

Biological Process: response to alkaloid; COPII coating of Golgi vesicle; intracellular protein transport; regulated secretory pathway; vesicle targeting, to, from or within Golgi; ER to Golgi vesicle-mediated transport; COPI coating of Golgi vesicle; retrograde vesicle-mediated transport, Golgi to ER; kidney development; Golgi organization and biogenesis; protein oligomerization