mutM recombinant protein

mutM, 1-269aa, E Coli, His tag, E Coli

Cat. Num. AAA204686

• Gene Names: mutM; ECK3625; fpg; JW3610
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: mutM; 1-269aa; E Coli; His tag; Formamidopyrimidine-DNA glycosylase; fpg; mutM recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK GEPCRVCGTP IVATKHAQRA TFYCRQCQK
• Sequence Length: 269

• Applicable Applications for mutM recombinant protein: SDS-PAGE
• Antigen Species: E Coli
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for mutM recombinant protein

mutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo. Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for mutM recombinant protein

Enzymes & Proteases

References

Serre L. et al. (2002) EMBO J. 21:2854-2865; Schalow BJ. et al. (2011) J Mol Biol, 410:183-193;

NCBI and Uniprot Product Information

• NCBI GI #: 16131506
• NCBI GeneID: 946765
• NCBI Accession #: NP_418092
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P05523; Q2M7U9
• Molecular Weight: 32.4 kDa (289aa), confirmed by MALDI-TOF
• NCBI Official Full Name: formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase
• NCBI Official Symbol: mutM
• NCBI Official Synonym Symbols: ECK3625; fpg; JW3610
• NCBI Protein Information: formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase
• UniProt Protein Name: Formamidopyrimidine-DNA glycosylase
• UniProt Gene Name: mutM
• UniProt Synonym Gene Names: fpg; Fapy-DNA glycosylase; AP lyase MutM

NCBI Description

MutM (also known as Fpg) is a DNA glycosylase that works in the base excision repair (BER) pathway to repair certain types of pre-mutagenic lesions. [More information is available at EcoCyc: EG10329].

Uniprot Description

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) and its derivatives such as guanidinohydantoin:C and spiroiminodihydantoin:C, however it also acts on thymine glycol:G, 5,6-dihydrouracil:G and 5-hydroxyuracil:G. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site.