Microsomal Triglyceride Transfer Protein Recombinant Protein | MTTP recombinant protein

Recombinant Microsomal Triglyceride Transfer Protein (MTTP)

Cat. Num. AAA2029259

• Gene Names: MTTP; ABL; MTP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Microsomal Triglyceride Transfer Protein; Recombinant Microsomal Triglyceride Transfer Protein (MTTP); MTTP recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; LNN ERLYKLTYST EVFLDGGKGK PQDSVGYKIS SDVDVVLLWR NPDGDDDQVI QVTITAVNVE NAGQQRGEKS IFQGKSTPKI IGKDNLEALQ RPMLLHLVRG KVKEFYSYEN EPVGIENLKR GLASLFQMQL SSGTTNEVDI SGDCKVTYQA QQDKVVKIKA LDTCKIERSG FTTANQVLGV SSKATSVTTY KIEDSFVTAV LAEETRAFAL NFQQTIAGKI VSKQKLELKT TEAGPRMIPG KQVAGVIKAV DSKYKAIPIV GQVLERVCKG CPSLAEHWKS IRKNLEPENL SKAEAVQSFL AFIQHLRTSR REEILQILKA EKKEVLPQLV DAVTSAQTPD SLEAILDFLD FKSDSSIILQ ERFLYACGFA THPDEELLRA LLSKFKGSFA SNDIRESVMI IIGALVRKLCQNEGCKLKAV VEAKKLILG
• Sequence Length: 20

• Applicable Applications for MTTP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 47.1kDa
• Accurate Molecular Mass (KD): 47kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Ser105
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4261846
• NCBI GeneID: 4547
• Molecular Weight: 16,865 Da
• NCBI Official Full Name: microsomal triglyceride transfer protein, partial
• NCBI Official Synonym Full Names: microsomal triglyceride transfer protein
• NCBI Official Symbol: MTTP
• NCBI Official Synonym Symbols: ABL; MTP
• NCBI Protein Information: microsomal triglyceride transfer protein large subunit
• UniProt Protein Name: Microsomal triglyceride transfer protein large subunit
• Protein Family: Microsomal triglyceride transfer protein
• UniProt Gene Name: MTTP
• UniProt Synonym Gene Names: MTP
• UniProt Entry Name: MTP_HUMAN

NCBI Description

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. [provided by RefSeq, Jul 2008]

Uniprot Description

MTTP: Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. Defects in MTTP are the cause of abetalipoproteinemia (ABL). ABL is an autosomal recessive disorder of lipoprotein metabolism. Affected individuals produce virtually no circulating apolipoprotein B-containing lipoproteins (chylomicrons, VLDL, LDL, lipoprotein(A)). Malabsorption of the antioxidant vitamin E occurs, leading to spinocerebellar and retinal degeneration.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: endoplasmic reticulum lumen; receptor complex

Molecular Function: lipid transporter activity; lipid binding

Biological Process: lipoprotein metabolic process; lipid metabolic process; lipid transport

Disease: Abdominal Obesity-metabolic Syndrome 1; Abetalipoproteinemia