Typical Testing Data/Standard Curve (for reference only)
Porcine Metallothionein 2 ELISA Kit | MT2 elisa kit
Porcine Metallothionein 2 ELISA Kit
Gene Names
MT2A; MT2
Reactivity
Porcine
Synonyms
Metallothionein 2; Porcine Metallothionein 2 ELISA Kit; MT2 elisa kit
Reactivity
Porcine
Samples
Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MT2 elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This MT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MT2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY MT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT2 antibody and an MT2-HRP conjugate. The assay sample and buffer are incubated together with MT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT2 concentration since MT2 from samples and MT2-HRP conjugate compete for the anti-MT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MT2 from the sample, fewer sites are left to bind MT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT2 concentration in each sample is interpolated from this standard curve.
INTENDED USE This MT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MT2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY MT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT2 antibody and an MT2-HRP conjugate. The assay sample and buffer are incubated together with MT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT2 concentration since MT2 from samples and MT2-HRP conjugate compete for the anti-MT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MT2 from the sample, fewer sites are left to bind MT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MT2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
6,042 Da
NCBI Official Full Name
metallothionein-2
NCBI Official Synonym Full Names
metallothionein 2A
NCBI Official Symbol
MT2A
NCBI Official Synonym Symbols
MT2
NCBI Protein Information
metallothionein-2; MT-2; MT-II; metallothionein-2A; metallothionein-II
UniProt Protein Name
Metallothionein-2
Protein Family
UniProt Gene Name
MT2A
UniProt Synonym Gene Names
CES1; MT2; MT-2; MT-II
UniProt Entry Name
MT2_HUMAN
Uniprot Description
MT2A: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. Belongs to the metallothionein superfamily. Type 1 family.
Protein type: Carrier
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; zinc ion binding; drug binding
Biological Process: cellular copper ion homeostasis; cytokine and chemokine mediated signaling pathway; negative regulation of growth
Research Articles on MT2
Similar Products
Product Notes
The Porcine MT2 mt2a (Catalog #AAA736996) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736996 ELISA Kit recognizes Porcine MT2. It is sometimes possible for the material contained within the vial of "Metallothionein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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