Selenoprotein X 1 Recombinant Protein | SEPX1 recombinant protein

Recombinant Human Selenoprotein X 1

Cat. Num. AAA144191

• Gene Names: MSRB1; SELR; SELX; SepR; SEPX1; HSPC270
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Selenoprotein X 1; Recombinant Human Selenoprotein X 1; SEPX1 Human; Selenoprotein X 1 Human Recombinant; Methionine-R-sulfoxide reductase B1; MsrB1; Selenoprotein X; SelX; SEPX1; SELR; SELX; HSPC270; MGC3344; SEPX1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The SEPX1 solution (0.5 mg/ml) contains 20mM Tris-HCl Buffer (pH 7.5), 1mM DTT, 0.1mM PMSF, 2mM EDTA and 10% Glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH
• Sequence Length: 116

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for SEPX1 recombinant protein

Description: SEPX1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 136 amino acids (1-116 a.a.) and having a molecular mass of 14.8kDa. In bacteria, the selenocystein (Sec/U) element is positioned directly following the UGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. The SEPX1 is purified by proprietary chromatographic techniques.

Introduction: Methionine sulfoxide reductase B1 (SEPX1 or MSRB1), is a selenoprotein that contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that usually signals translation termination. SEPX1 is a member of the methionine sulfoxide reductase B (MsrB) family, and is expressed in an assortment of adult and fetal tissues. MSRs (Methionine sulfoxide reductases) catalyze the reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS creates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two separate enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO.

Product Categories/Family for SEPX1 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7706511
• NCBI GeneID: 51734
• NCBI Accession #: NP_057416.1
• NCBI GenBank Nucleotide #: NM_016332.2
• UniProt Accession #: Q9NZV6; Q96RX6; Q9BTV2; Q9P0B1
• Molecular Weight: 12,760 Da
• NCBI Official Full Name: methionine-R-sulfoxide reductase B1
• NCBI Official Synonym Full Names: methionine sulfoxide reductase B1
• NCBI Official Symbol: MSRB1
• NCBI Official Synonym Symbols: SELR; SELX; SepR; SEPX1; HSPC270
• NCBI Protein Information: methionine-R-sulfoxide reductase B1; selenoprotein R; selenoprotein X, 1
• UniProt Protein Name: Methionine-R-sulfoxide reductase B1
• UniProt Gene Name: MSRB1
• UniProt Synonym Gene Names: SEPX1; MsrB1; SelX
• UniProt Entry Name: MSRB1_HUMAN

NCBI Description

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol. [provided by RefSeq, Mar 2012]

Uniprot Description

SEPX1: a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol. [provided by RefSeq, Mar 2012]

Protein type: EC 1.8.4.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nucleus; actin cytoskeleton

Molecular Function: zinc ion binding; peptide-methionine (R)-S-oxide reductase activity; actin binding

Biological Process: protein repair; actin filament polymerization; innate immune response; response to oxidative stress