SEPX1 recombinant protein

SEPX1, 1-116aa, Human, His tag, E Coli

Cat. Num. AAA203456

• Gene Names: MSRB1; SELR; SELX; SepR; SEPX1; HSPC270
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SEPX1; 1-116aa; Human; His tag; E Coli; Methionine-R-sulfoxide reductase B1; Selenoprotein X; HSPC270; MSRB1; SELR; SELX; u95C; SEPX1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH
• Sequence Length: 116

• Applicable Applications for SEPX1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SEPX1 recombinant protein

SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases (MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/u) element is located immediately following the uGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for SEPX1 recombinant protein

Redox Proteins

References

Gladdyshev VN., et al. (2010) PLoS One. 5 (7):e11497.; Thiele JJ., et al. (2009) Am J Dermatopathol. 31 (5):427-31.;

NCBI and Uniprot Product Information

• NCBI GI #: 7706511
• NCBI GeneID: 51734
• NCBI Accession #: NP_057416
• NCBI GenBank Nucleotide #: NM_016332.3
• UniProt Accession #: Q9NZV6; Q96RX6; Q9BTV2; Q9P0B1
• Molecular Weight: 14.8 kDa (136aa), confirmed by MALDI-TOF
• NCBI Official Full Name: methionine-R-sulfoxide reductase B1
• NCBI Official Synonym Full Names: methionine sulfoxide reductase B1
• NCBI Official Symbol: MSRB1
• NCBI Official Synonym Symbols: SELR; SELX; SepR; SEPX1; HSPC270
• NCBI Protein Information: methionine-R-sulfoxide reductase B1
• UniProt Protein Name: Methionine-R-sulfoxide reductase B1
• UniProt Gene Name: MSRB1
• UniProt Synonym Gene Names: SEPX1; MsrB1; SelX

NCBI Description

The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 19. [provided by RefSeq, Aug 2017]

Uniprot Description

Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by MICALs (MICAL1, MICAL2 or MICAL3) on actin, thereby promoting filament repolymerization. Plays a role in innate immunity by reducing oxidized actin, leading to actin repolymerization in macrophages.