MSR1 / SCARA1 recombinant protein

Recombinant Rat MSR1 / SCARA1 Protein (Fc tag)

Cat. Num. AAA2547320

• Gene Names: MSR1; SRA; SR-A; CD204; phSR1; phSR2; SCARA1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: MSR1 / SCARA1; Recombinant Rat MSR1 / SCARA1 Protein (Fc tag); MSR1; MSR1 / SCARA1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 388

• Application Notes: The recombinant Rat MSR1/Fc comprises 636 amino acids and has a predicted molecular mass of 69.2 kDa. The apparent molecular mass of the protein is approximately 89 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MSR1 / SCARA1 recombinant protein

Background: Macrophage scavenger receptor type s I and II, also known as Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1, CD204, MSR1 and SCARA1, is a single-pass type I I membrane protein which contains one collagen-like domain and one SRCR domain. Macrophages are distributed in all peripheral tissues and play a critical role in the first line of the innate immune defenses against bacterial infection by phagocytosis of bacterial pathogens through the macrophage scavenger receptor 1 (MSR1). MSR1 / SCARA1 is one of the membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two type s of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). MSR1 / SCARA1 is also involved in chronic inflammation which is a risk factor for prostate cancer. MSR1 1 gene was identified as a candidate susceptibility gene for hereditary prostate cancer and as a risk factor for sporadic prostate cancer.

Description: A DNA sequence encoding the rat MSR1 (Trp79-Ser454) was expressed, fused with the Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 20357515
• NCBI GeneID: 4481
• NCBI Accession #: NP_619730.1
• NCBI GenBank Nucleotide #: NM_138716.2
• UniProt Accession #: P21757; O60505; P21759; Q45F10; D3DSP3
• Molecular Weight: 42,942 Da
• NCBI Official Full Name: macrophage scavenger receptor types I and II isoform type 3
• NCBI Official Synonym Full Names: macrophage scavenger receptor 1
• NCBI Official Symbol: MSR1
• NCBI Official Synonym Symbols: SRA; SR-A; CD204; phSR1; phSR2; SCARA1
• NCBI Protein Information: macrophage scavenger receptor types I and II
• UniProt Protein Name: Macrophage scavenger receptor types I and II
• UniProt Gene Name: MSR1
• UniProt Synonym Gene Names: SCARA1

NCBI Description

This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]

Uniprot Description

MSR1: Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. Defects in MSR1 may be a cause of prostate cancer (PC). A malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet- ring cell carcinoma and neuroendocrine carcinoma. MSR1 variants may play a role in susceptibility to prostate cancer. MSR1 variants have been found in individuals with prostate cancer and co-segregate with the disease in some families. Defects in MSR1 may be a cause of Barrett esophagus (BE). A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes. Genetic variants in MSR1 have been found in individuals with Barrett esophagus and are thought to contribute to disease susceptibility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: low-density lipoprotein binding; protein binding

Biological Process: cholesterol transport; receptor-mediated endocytosis

Disease: Barrett Esophagus; Prostate Cancer