Typical Testing Data/Standard Curve (for reference only)
Monkey Macrophage stimulating protein ELISA Kit | MSP elisa kit
Monkey Macrophage stimulating protein ELISA Kit
Gene Names
MST1; MSP; HGFL; NF15S2; D3F15S2; DNF15S2
Reactivity
Monkey
Synonyms
Macrophage stimulating protein; Monkey Macrophage stimulating protein ELISA Kit; MSP elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of MSP. No significant cross-reactivity or interference between MSP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MSP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 mg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MSP elisa kit
Intended Uses: This MSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey MSP. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: MSP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MSP antibody and an MSP-HRP conjugate. The assay sample and buffer are incubated together with MSP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSP concentration since MSP from samples and MSP-HRP conjugate compete for the anti-MSP antibody binding site. Since the number of sites is limited, as more sites are occupied by MSP from the sample, fewer sites are left to bind MSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MSP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MSP antibody and an MSP-HRP conjugate. The assay sample and buffer are incubated together with MSP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MSP concentration since MSP from samples and MSP-HRP conjugate compete for the anti-MSP antibody binding site. Since the number of sites is limited, as more sites are occupied by MSP from the sample, fewer sites are left to bind MSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MSP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MSP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
80,320 Da
NCBI Official Full Name
macrophage-stimulating protein
NCBI Official Synonym Full Names
macrophage stimulating 1 (hepatocyte growth factor-like)
NCBI Official Symbol
MST1
NCBI Official Synonym Symbols
MSP; HGFL; NF15S2; D3F15S2; DNF15S2
NCBI Protein Information
hepatocyte growth factor-like protein; macrophage-stimulating protein; hepatocyte growth factor-like protein homolog
UniProt Protein Name
Hepatocyte growth factor-like protein
Protein Family
UniProt Gene Name
MST1
UniProt Synonym Gene Names
D3F15S2; DNF15S2; HGFL; MSP
UniProt Entry Name
HGFL_HUMAN
NCBI Description
The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq, Jan 2010]
Uniprot Description
MSP: Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. Belongs to the peptidase S1 family. Plasminogen subfamily.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3p21
Molecular Function: serine-type endopeptidase activity
Biological Process: sperm motility; spermatogenesis; proteolysis; embryo implantation
Research Articles on MSP
Similar Products
Product Notes
The Monkey MSP mst1 (Catalog #AAA750396) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA750396 ELISA Kit recognizes Monkey MSP. It is sometimes possible for the material contained within the vial of "Macrophage stimulating protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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