WD-40 repeat-containing protein MSI1 (MSI1) Recombinant Protein | MSI1 recombinant protein

Recombinant Arabidopsis thaliana WD-40 repeat-containing protein MSI1 (MSI1)

Cat. Num. AAA1185653

• Gene Names: MSI1; ARABIDOPSIS MULTICOPY SUPRESSOR OF IRA1; ATMSI1; MATERNAL EFFECT EMBRYO ARREST 70; MCK7.10; MCK7_10; MEE70; MULTICOPY SUPRESSOR OF IRA1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: WD-40 repeat-containing protein MSI1 (MSI1); Recombinant Arabidopsis thaliana WD-40 repeat-containing protein MSI1 (MSI1); MSI1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-424, Full length protein
• Sequence: MGKDEEEMRGEIEERLINEEYKIWKKNTPFLYDLVITHALEWPSLTVEWLPDREEPSGKDYSVQKMILGTHTSESEPNYLMLAQVQLPLDDTESEARQYDDDRSEFGGFGCATGKVQIIQQINHDGEVNRARYMPQNPFIIATKTVNAEVYVFDYSKHPSKPPLDGACNPDLKLRGHSSEGYGLSWSKFKQGHLLSGSDDAQICLWDINATPKNKSLDAQQIFKAHEGVVEDVAWHLRHEYLFGSVGDDQYLLIWDLRSPSASKPVQSVVAHSMEVNCLAFNPFNEWVVATGSTDKTVKLFDLRKLSTALHTFDSHKEEVFQVGWNPKNETILASCCLGRRLMVWDLSRIDEEQTVEDAEDGPPELLFIHGGHTSKISDFSWNPCEDWVISSVAEDNILQIWQMAENIYHDEDDAPGEEPSKAS
• Sequence Length: 424

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15237140
• NCBI GeneID: 835935
• NCBI Accession #: NP_200631.1
• NCBI GenBank Nucleotide #: NM_125208.3
• UniProt Accession #: O22467
• Molecular Weight: 48,195 Da
• NCBI Official Full Name: Transducin/WD40 repeat-like superfamily protein
• NCBI Official Symbol: MSI1
• NCBI Official Synonym Symbols: ARABIDOPSIS MULTICOPY SUPRESSOR OF IRA1; ATMSI1; MATERNAL EFFECT EMBRYO ARREST 70; MCK7.10; MCK7_10; MEE70; MULTICOPY SUPRESSOR OF IRA1
• NCBI Protein Information: Transducin/WD40 repeat-like superfamily protein
• UniProt Protein Name: Histone-binding protein MSI1
• Protein Family: WD-40 repeat-containing protein
• UniProt Gene Name: MSI1
• UniProt Synonym Gene Names: CAF-1 subunit MSI1; AtMSI1

NCBI Description

Encodes a WD-40 repeat containing protein that functions in chromatin assembly as part of the CAF1 and FIE complex. Mutants exhibit parthenogenetic development that includes proliferation of unfertilized endosperm and embryos. In heterozygous plants 50% of embryos abort. Of the aborted embryos the early aborted class are homozygous and the later aborting lass are heterozygotes in which the defective allele is maternally transmitted. Other phenotypes include defects in ovule morphogenesis and organ initiation,as well as increased levels of heterochromatic DNA. MSI1 is needed for the transition to flowering. In Arabidopsis, the three CAF-1 subunits are encoded by FAS1, FAS2 and, most likely, MSI1, respectively. Mutations in FAS1 or FAS2 lead to increased frequency of homologous recombination and T-DNA integration in Arabidopsis. In the ovule, the MSI1 transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization. MSI is biallelically expressed, the paternall allele is expressed in the endosperm and embryo and is not imprinted. MSI1 forms a complex with RBR1 that is required for activation of the imprinted genes FIS2 and FWA. This activation is mediated by MSI1/RBR1 mediated repression of MET1.

Uniprot Description

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair, and the fertilization independent seed (FIS) complex, a polycomb group protein complex which is required to maintain the transcriptionally repressive state of homeotic genes throughout development. Required for several aspects of plant development including normal leaf expansion, correct development of flowers, normal endosperm development, repression of parthenogenetic seed development and repression of floral homeotic genes in leaf tissue. Also required for the recruitment of chromosomal DNA into heterochromatic chromocenters. Also involved in regulation of imprinted genes. Acts together with RBR1 to repress the expression of MET1 during female gametogenesis. This in turn activates expression of the imprinted genes FIS2 and FWA.