Protein Zero, Myelin (MPZ) Recombinant Protein | MPZ recombinant protein

Recombinant Protein Zero, Myelin (MPZ)

Cat. Num. AAA2010939

• Gene Names: MPZ; P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Zero; Myelin (MPZ); Recombinant Protein Zero; MPZ recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- I VVYTDREVHG AVGSRVTLHC SFWSSEWVSD DISFTWRYQP EGGRDAISIF HYAKGQPYID EVGTFKERIQ WVGDPRWKDG SIVIHNLDYS DNGTFTCDVK NPPDIVGKTS QVTLYVFEKV PTRYGVVLGA VIGGVLGVVL LLLLLFYVVR YCWLRRQAAL QRRLSAMEKG KLHKPGKDAS KRGRQTPVLY AMLDHSRSTK AVSEKKAKGL GESRKDKK
• Sequence Length: 248

• Applicable Applications for MPZ recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ile30~Lys248 (Accession # P25189) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 295391071
• NCBI GeneID: 4359
• NCBI Accession #: NP_000521.2
• NCBI GenBank Nucleotide #: NM_000530.6
• UniProt Accession #: P25189; Q16072; Q5VTH4; Q92677; Q9BR67
• Molecular Weight: 26.3kDa
• NCBI Official Full Name: myelin protein P0
• NCBI Official Synonym Full Names: myelin protein zero
• NCBI Official Symbol: MPZ
• NCBI Official Synonym Symbols: P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
• NCBI Protein Information: myelin protein P0; myelin peripheral protein; Charcot-Marie-Tooth neuropathy 1B
• UniProt Protein Name: Myelin protein P0
• Protein Family: Myelin protein
• UniProt Gene Name: MPZ
• UniProt Synonym Gene Names: MPP
• UniProt Entry Name: MYP0_HUMAN

NCBI Description

This gene encodes a major structural protein of peripheral myelin. Mutations in this gene result in the autosomal dominant form of Charcot-Marie-Tooth disease type 1 and other polyneuropathies. [provided by RefSeq, Apr 2010]

Uniprot Description

myelin P0: a structural protein in peripheral nervous system Schwann cells. Forms an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. Defects cause of Charcot-Marie-Tooth disease, Dejerine-Sottas syndrome, congenital hypomyelination neuropathy, and Roussy-Levy syndrome.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to plasma membrane; plasma membrane; myelin sheath

Molecular Function: structural molecule activity

Biological Process: synaptic transmission; intercellular junction maintenance

Disease: Neuropathy, Congenital Hypomyelinating Or Amyelinating, Autosomal Recessive; Charcot-marie-tooth Disease, Demyelinating, Type 1b; Hypertrophic Neuropathy Of Dejerine-sottas; Charcot-marie-tooth Disease, Dominant Intermediate D; Charcot-marie-tooth Disease, Axonal, Type 2j; Charcot-marie-tooth Disease, Axonal, Type 2i; Roussy-levy Hereditary Areflexic Dystasia