SDS-PAGE
Glyceraldehyde-3-phosphate dehydrogenase Recombinant Protein | GAPDH recombinant protein
Recombinant Gallus gallus Glyceraldehyde-3-phosphate dehydrogenase
Gene Names
GAPDH; KNC-NDS6
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Glyceraldehyde-3-phosphate dehydrogenase; Recombinant Gallus gallus Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-); GAPDH recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
2-333aa; Full Length
Sequence
VKVGVNGFGRIGRLVTRAAVLSGKVQVVAINDPFIDLNYMVYMFKYDSTHGHFKGTVKAENGKLVINGHAITIFQERDPSNIKWADAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDKSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVVKAAADGPLKGILGYTEDQVVSCDFNGDSHSSTFDAGAGIALNDHFVKLVSWYDNEFGYSNRVVDLMVHMASKE
Sequence Length
333
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for GAPDH recombinant protein
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins.
References
Complete nucleotide sequence of the messenger RNA coding for chicken muscle glyceraldehyde-3-phosphate dehydrogenase.Panabieres F., Piechaczyk M., Rainer B., Dani C., Fort P., Riaad S., Marty L., Imbach J.L., Jeanteur P., Blanchard J.-M.Biochem. Biophys. Res. Commun. 118:767-773(1984)
Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle.Dugaiczyk A., Haron J.A., Stone E.M., Dennison O.E., Rothblum K.N., Schwartz R.J.Biochemistry 22:1605-1613(1983)
Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene.Stone E.M., Rothblum K.N., Alevy M.C., Kuo T.M., Schwartz R.J.Proc. Natl. Acad. Sci. U.S.A. 82:1628-1632(1985)
Several novel transcripts of glyceraldehyde-3-phosphate dehydrogenase expressed in adult chicken testis.Mezquita J., Pau M., Mezquita C.3.0.CO;2-K>J. Cell. Biochem. 71:127-139(1998)
Cloning, partial sequencing, and expression of glyceraldehyde-3-phosphate dehydrogenase gene in chick embryonic heart muscle cells.Arnold H.H., Domdey H., Wiebauer K., Datta K., Siddiqui M.A.Q.J. Biol. Chem. 257:9872-9877(1982)
ErratumArnold H.H., Domdey H., Wiebauer K., Datta K., Siddiqui M.A.Q.J. Biol. Chem. 258:2063-2063(1983)
Glyceraldehyde 3-phosphate dehydrogenase protein and mRNA are both differentially expressed in adult chickens but not chick embryos.Milner R.J., Brow M.A.D., Cleveland D.W., Shinnick T.M., Sutcliff J.G.Nucleic Acids Res. 11:3301-3315(1983)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39.6 kDa
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
KNC-NDS6
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
Protein Family
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_CHICK
Uniprot Description
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins ().
Research Articles on GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA962931) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-333aa; Full Length. The amino acid sequence is listed below: VKVGVNGFGR IGRLVTRAAV LSGKVQVVAI NDPFIDLNYM VYMFKYDSTH GHFKGTVKAE NGKLVINGHA ITIFQERDPS NIKWADAGAE YVVESTGVFT TMEKAGAHLK GGAKRVIISA PSADAPMFVM GVNHEKYDKS LKIVSNASCT TNCLAPLAKV IHDNFGIVEG LMTTVHAITA TQKTVDGPSG KLWRDGRGAA QNIIPASTGA AKAVGKVIPE LNGKLTGMAF RVPTPNVSVV DLTCRLEKPA KYDDIKRVVK AAADGPLKGI LGYTEDQVVS CDFNGDSHSS TFDAGAGIAL NDHFVKLVSW YDNEFGYSNR VVDLMVHMAS KE. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-phosphate dehydrogenase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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