CD110 recombinant protein

Recombinant Human CD110 protein, C-His Tag

Cat. Num. AAA1560568

• Gene Names: MPL; MPLV; TPOR; C-MPL; CD110; THCYT2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD110; Recombinant Human CD110 protein; C-His Tag; CD110 antigen; c-Mpl; MPL; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; Thrombopoietin R; thrombopoietin receptor; TpoR; TPO-R; TPORMPLV; CD110 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Tag: C-His Tag
• Species: Homo sapiens (Human)
• Endotoxin Level: <1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the human MPL (Met1-Trp491) was fused with the C-terminal His Tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

SDS-Page

Related Product Information for CD110 recombinant protein

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95kDa type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily. The 635 amino acid (aa) full-length human Tpo R contains a 25 aa signal sequence, a 466 aa extracellular domain with a ligand binding domain and two fibronectin type III domains, a transmembrane (TM) domain and a cytoplasmic domain. The extracellular domain of human Tpo R shares 78, 76, 81, 82 and 80 aa identity with mouse, rat, bovine, canine and equine Tpo R, respectively. Humans produce three distinct mRNA species; a P-form, a K-form, and a truncated form (Mpl-tr) lacking a TM domain. The P-form encodes the full-length receptor. The Mpl-tr form, which is expressed in both human and mouse, is intracellular and targets the P-form for degradation. The 579 aa K-form has an alternate cytoplasmic domain, but does not dimerize with, or inhibit, the P-form. Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis, thrombopoiesis and hematopoietic stem cell self-renewal, as reflected by expression of the Tpo R on megakaryocytes, platelets and hematopoietic progenitors. Receptor dimerization occurs upon Tpo binding and initiates signaling through the Ras/MAP and JAK/STAT pathways. Internalization and degradation of Tpo following Tpo R binding serves to downregulate circulating Tpo. Tpo R expressed at low levels on endothelial cells does not appear to contribute to regulation of Tpo. Inactivating mutations of Tpo R cause thrombocytopenia, and absence of functional Tpo R is lethal in humans, but not mice. Other mutations, including an activating change in the TM domain, can cause thrombocytosis.

Product Categories/Family for CD110 recombinant protein

Protein

References

Kaushansky, K. (2005) J. Clin. Invest. 115:3339.

NCBI and Uniprot Product Information

• NCBI GI #: 4885491
• NCBI GeneID: 4352
• NCBI Accession #: NP_005364.1
• NCBI GenBank Nucleotide #: NM_005373.2
• UniProt Accession #: P40238; Q5JUZ0
• Molecular Weight: 65,521 Da
• NCBI Official Full Name: thrombopoietin receptor
• NCBI Official Synonym Full Names: MPL proto-oncogene, thrombopoietin receptor
• NCBI Official Symbol: MPL
• NCBI Official Synonym Symbols: MPLV; TPOR; C-MPL; CD110; THCYT2
• NCBI Protein Information: thrombopoietin receptor; TPO-R; proto-oncogene c-Mpl; myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene
• UniProt Protein Name: Thrombopoietin receptor
• UniProt Gene Name: MPL
• UniProt Synonym Gene Names: TPOR; TPO-R

NCBI Description

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008]

Uniprot Description

MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cell surface; Golgi apparatus; integral component of plasma membrane; plasma membrane

Molecular Function: protein binding; thrombopoietin receptor activity; transmembrane receptor activity

Biological Process: cell proliferation; cell surface receptor signaling pathway; thrombopoietin-mediated signaling pathway

Disease: Amegakaryocytic Thrombocytopenia, Congenital; Myelofibrosis; Thrombocythemia 2