Mouse beta-Tubulin Antibody | anti-TUBB antibody

Anti-beta-Tubulin Purified

Cat. Num. AAA1801622

• Reactivity: Mouse, Rat, Pig, Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Purified by Precipitation and Chromatography
• Synonyms: beta-Tubulin; Anti-beta-Tubulin Purified; tubulin beta; TUBB; anti-TUBB antibody

• Host: Mouse
• Reactivity: Mouse, Rat, Pig, Human
• Isotype: IgM
• Clone Number: TU-12
• Specificity: The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.
• Purity/Purification: Purified by Precipitation and Chromatography
• Form/Format: Tris buffered saline (TBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-TUBB antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen: Porcine brain microtubule protein MTP-1.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for anti-TUBB antibody

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

NCBI and Uniprot Product Information

• NCBI GI #: 13562114
• NCBI GeneID: 81027
• NCBI Accession #: NP_110400
• NCBI GenBank Nucleotide #: NP_110400.1
• UniProt Accession #: Q9H4B7
• NCBI Official Full Name: tubulin beta-1 chain
• NCBI Official Synonym Full Names: tubulin beta 1 class VI
• NCBI Official Symbol: TUBB1
• NCBI Protein Information: tubulin beta-1 chain
• UniProt Protein Name: Tubulin beta-1 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB1
• UniProt Entry Name: TBB1_HUMAN

NCBI Description

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010]