ANO6 sirna

ANO6 siRNA (Mouse)

Cat. Num. AAA8241070

• Gene Names: Ano6; Tmem16f; AA407480; AW554778; 2900059G15Rik; F730003B03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ANO6; ANO6 siRNA (Mouse); TMEM16F; Anoctamin-6; Small-conductance calcium-activated nonselective cation channel; SCAN channel; Transmembrane protein 16F; ANO6 sirna

• Reactivity: Mouse
• Specificity: ANO6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 484

• Applicable Applications for ANO6 sirna: RNA Interference (RNAi)
• Source: Synthetic
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ANO6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4°C. Store at -20°C for one year.

Related Product Information for ANO6 sirna

siRNA to inhibit ANO6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 359465539
• NCBI GeneID: 105722
• NCBI Accession #: NP_001240742.1
• NCBI GenBank Nucleotide #: NM_001253813.1
• UniProt Accession #: Q6P9J9; Q8C242
• NCBI Official Full Name: anoctamin-6 isoform 1
• NCBI Official Synonym Full Names: anoctamin 6
• NCBI Official Symbol: Ano6
• NCBI Official Synonym Symbols: Tmem16f; AA407480; AW554778; 2900059G15Rik; F730003B03Rik
• NCBI Protein Information: anoctamin-6
• UniProt Protein Name: Anoctamin-6
• Protein Family: Anoctamin
• UniProt Gene Name: Ano6
• UniProt Synonym Gene Names: Tmem16f; SCAN channel
• UniProt Entry Name: ANO6_MOUSE

Uniprot Description

ANO6: May act as a calcium-activated chloride channel. It is essential for calcium-dependent exposure of phosphatidylserine on the surface of activated platelets, a process necessary to trigger the clotting system. Defects in ANO6 are the cause of Scott syndrome (SCTS). A mild bleeding disorder due to impaired surface exposure of procoagulant phosphatidylserine (PS) on platelets and other blood cells, following activation with Ca(2+)-elevating agents. Belongs to the anoctamin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; intracellular

Molecular Function: phospholipid scramblase activity; chloride channel activity; protein homodimerization activity; intracellular calcium activated chloride channel activity; voltage-gated chloride channel activity; voltage-gated ion channel activity; calcium activated cation channel activity

Biological Process: activation of blood coagulation via clotting cascade; transport; dendritic cell chemotaxis; ion transport; phospholipid transport; chloride transport; phospholipid scrambling; lipid transport; blood coagulation; cation transport