Mouse Optineurin (OPTN) RTU ELISA Kit | OPTN rtu elisa kit

Mouse Optineurin (OPTN) ELISA Kit

Cat. Num. AAA4501588

• Gene Names: Optn; NRP; FIP2; HYPL; 4930441O07Rik
• Reactivity: Mouse
• Synonyms: Optineurin (OPTN); Mouse Optineurin (OPTN) ELISA Kit; NRP; FIP2; GLC1E; HIP7; HYPL; TFIIIA-INTP; Glaucoma 1; Open Angle; E(Adult-Onset); Huntingtin-interacting protein 7; Optic neuropathy-inducing protein; E3-14.7K-interacting protein; OPTN rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 584

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPTN rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OPTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OPTN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32469491
• NCBI GeneID: 71648
• NCBI Accession #: NP_862896.1
• NCBI GenBank Nucleotide #: NM_181848.5
• UniProt Accession #: Q8K3K8; A2ASP3
• Molecular Weight: 67,018 Da
• NCBI Official Full Name: optineurin
• NCBI Official Synonym Full Names: optineurin
• NCBI Official Symbol: Optn
• NCBI Official Synonym Symbols: NRP; FIP2; HYPL; 4930441O07Rik
• NCBI Protein Information: optineurin
• UniProt Protein Name: Optineurin
• Protein Family: Optineurin
• UniProt Gene Name: Optn

Uniprot Description

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.