Mouse Kininogen 1 (KNG1) RTU ELISA Kit | KNG1 rtu elisa kit

Mouse Kininogen 1 (KNG1) ELISA Kit

Cat. Num. AAA4500634

• Gene Names: Kng1; Kng
• Reactivity: Mouse
• Synonyms: Kininogen 1 (KNG1); Mouse Kininogen 1 (KNG1) ELISA Kit; BDK; KNG; HMWK; Alpha-2-Thiol Proteinase Inhibitor; Bradykinin; Kallidin; Fitzgerald factor; High molecular weight kininogen; Williams-Fitzgerald-Flaujeac factor; Ile-Ser-Bradykinin; KNG1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 661

• Assay Type: Sandwich
• Samples: Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.276ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KNG1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KNG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KNG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KNG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KNG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 156231021
• NCBI GeneID: 16644
• NCBI Accession #: NP_001095881.1
• NCBI GenBank Nucleotide #: NM_001102411.1
• UniProt Accession #: O08677; O08676; Q32MX7; Q6S9I1; Q91XK5
• Molecular Weight: 53,206 Da
• NCBI Official Full Name: kininogen-1 isoform 1
• NCBI Official Synonym Full Names: kininogen 1
• NCBI Official Symbol: Kng1
• NCBI Official Synonym Symbols: Kng
• NCBI Protein Information: kininogen-1
• UniProt Protein Name: Kininogen-1
• Protein Family: Kininogen
• UniProt Gene Name: Kng1
• UniProt Synonym Gene Names: Kng

Uniprot Description

1 Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting ().