Mouse Hypoxia Inducible Factor 1 Alpha (HIF1a) RTU ELISA Kit | HIF1a rtu elisa kit

Mouse Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

Cat. Num. AAA459858

• Gene Names: Hif1a; MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
• Reactivity: Mouse
• Synonyms: Hypoxia Inducible Factor 1 Alpha (HIF1a); Mouse Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit; HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; HIF1a rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 837

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF1a rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF1a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HIF1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 927028881
• NCBI GeneID: 15251
• NCBI Accession #: NP_001300848.1
• NCBI GenBank Nucleotide #: NM_001313919.1
• UniProt Accession #: Q61221; O08741; O08993; Q61664; Q61665; Q8C681; Q8CC19; Q8CCB6; Q8R385; Q9CYA8
• Molecular Weight: 91,874 Da
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1, alpha subunit
• NCBI Official Symbol: Hif1a
• NCBI Official Synonym Symbols: MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: Hif1a
• UniProt Synonym Gene Names: HIF-1-alpha; HIF1-alpha

NCBI Description

This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia ().