Mouse Cofilin 1, Non Muscle (CFL1) RTU ELISA Kit | CFL1 rtu elisa kit

Mouse Cofilin 1, Non Muscle (CFL1) ELISA Kit

Cat. Num. AAA458333

• Gene Names: Cfl1; Cof; AA959946
• Reactivity: Mouse
• Synonyms: Cofilin 1; Non Muscle (CFL1); Mouse Cofilin 1; Non Muscle (CFL1) ELISA Kit; CFL; p18; N-Cofilin; 18 kDa phosphoprotein; CFL1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 166

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.108ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFL1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CFL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CFL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CFL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CFL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6680924
• NCBI GeneID: 12631
• NCBI Accession #: NP_031713.1
• NCBI GenBank Nucleotide #: NM_007687.5
• UniProt Accession #: P18760
• Molecular Weight: 18,560 Da
• NCBI Official Full Name: cofilin-1
• NCBI Official Synonym Full Names: cofilin 1, non-muscle
• NCBI Official Symbol: Cfl1
• NCBI Official Synonym Symbols: Cof; AA959946
• NCBI Protein Information: cofilin-1
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: Cfl1

Uniprot Description

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11809832). Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (). Required for neural tube morphogenesis and neural crest cell migration (PubMed:15649475).