Mouse Bone Morphogenetic Protein 8B (BMP8B) RTU ELISA Kit | BMP8B rtu elisa kit

Mouse Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit

Cat. Num. AAA457958

• Gene Names: Bmp8b; Op3
• Reactivity: Mouse
• Synonyms: Bone Morphogenetic Protein 8B (BMP8B); Mouse Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit; BMP8-B; OP2; Osteogenic Protein 2; BMP8B rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 399

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.1pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP8B rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP8B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BMP8B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMP8B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMP8B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31560733
• NCBI GeneID: 12164
• NCBI Accession #: NP_031585.2
• NCBI GenBank Nucleotide #: NM_007559.5
• UniProt Accession #: P55105; Q8BNM2
• Molecular Weight: 44,943 Da
• NCBI Official Full Name: bone morphogenetic protein 8B preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 8b
• NCBI Official Symbol: Bmp8b
• NCBI Official Synonym Symbols: Op3
• NCBI Protein Information: bone morphogenetic protein 8B
• UniProt Protein Name: Bone morphogenetic protein 8B
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp8b
• UniProt Synonym Gene Names: BMP-8B

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The encoded protein may play a role in the generation of primordial germ cells, and has been shown to stimulate thermogenesis in brown adipose tissue. Male mice lacking a functional copy of this gene exhibit variable degrees of germ-cell deficiency. Homozygous knockout mice of both sexes exhibit impaired thermogenesis and reduced metabolic rate, resulting in weight gain. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (). Involved in the generation of primordial germ cells; this function involves Bmp4 in a synergistic manner though separate receptor complexes seem to be involved. Required for the initiation and maintenance of spermatogenesis. Signaling protein involved in regulation of thermogenesis and energy balance. Proposed to increase the peripheral response of brown adipose tissue (BAT) to adrenergic stimulation while acting centrally in the hypothalamus to increase sympathetic output to BAT.