Mouse Autoimmune Regulator (AIRE) RTU ELISA Kit | AIRE rtu elisa kit

Mouse Autoimmune Regulator (AIRE) ELISA Kit

Cat. Num. AAA457513

• Reactivity: Mouse
• Synonyms: Autoimmune Regulator (AIRE); Mouse Autoimmune Regulator (AIRE) ELISA Kit; AIRE1; APECED; APS1; APSI; PGA1; Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy; AIRE rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 551

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AIRE rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AIRE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AIRE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AIRE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AIRE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 409168278
• NCBI GeneID: 11634
• NCBI Accession #: NP_001258478.1
• NCBI GenBank Nucleotide #: NM_001271549.1
• UniProt Accession #: Q9Z0E3; Q9JLW0; Q9JLW1; Q9JLW2; Q9JLW3; Q9JLW4; Q9JLW5; Q9JLW6; Q9JLW7; Q9JLW8; Q9JLW9; Q9JLX0
• Molecular Weight: 44,032 Da
• NCBI Official Full Name: autoimmune regulator isoform 2
• NCBI Official Synonym Full Names: autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
• NCBI Official Symbol: Aire
• NCBI Protein Information: autoimmune regulator
• UniProt Protein Name: Autoimmune regulator
• Protein Family: Autoimmune regulator
• UniProt Gene Name: Aire
• UniProt Synonym Gene Names: APECED protein homolog

Uniprot Description

Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (Probable). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program (PubMed:19015306). Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression (PubMed:21300913). Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes (PubMed:19923453). In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection (PubMed:26070482). In secondary lymphoid organs, induces functional inactivation of CD4+ T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli (PubMed:23993652).