Human Autoimmune Regulator (AIRE) ELISA Kit | AIRE elisa kit

Human Autoimmune Regulator (AIRE) ELISA Kit

Cat. Num. AAA2704364

• Gene Names: AIRE; APS1; APSI; PGA1; AIRE1; APECED
• Reactivity: Human
• Synonyms: Autoimmune Regulator (AIRE); Human Autoimmune Regulator (AIRE) ELISA Kit; AIRE1; APECED; APS1; APSI; PGA1; Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy; AIRE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Autoimmune Regulator (AIRE).; No significant cross-reactivity or interference between Autoimmune Regulator (AIRE) and analogues was observed.
• Sequence Length: 348

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autoimmune Regulator (AIRE) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autoimmune Regulator (AIRE) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AIRE elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autoimmune Regulator (AIRE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autoimmune Regulator (AIRE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autoimmune Regulator (AIRE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autoimmune Regulator (AIRE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557291
• NCBI GeneID: 326
• NCBI Accession #: NP_000374.1
• NCBI GenBank Nucleotide #: NM_000383.3
• UniProt Accession #: O43918; O43922; O43932; O75745; B2RP50
• Molecular Weight: 36,501 Da
• NCBI Official Full Name: autoimmune regulator
• NCBI Official Synonym Full Names: autoimmune regulator
• NCBI Official Symbol: AIRE
• NCBI Official Synonym Symbols: APS1; APSI; PGA1; AIRE1; APECED
• NCBI Protein Information: autoimmune regulator
• UniProt Protein Name: Autoimmune regulator
• Protein Family: Autoimmune regulator
• UniProt Gene Name: AIRE
• UniProt Synonym Gene Names: APECED; APECED protein

NCBI Description

This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012]

Uniprot Description

Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (PubMed:26084028). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (PubMed:26084028). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4+ T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli ().