Mouse Androgen Receptor (AR) RTU ELISA Kit | AR rtu elisa kit

Mouse Androgen Receptor (AR) ELISA Kit

Cat. Num. AAA457763

• Gene Names: Ar; Tfm; AW320017
• Reactivity: Mouse
• Synonyms: Androgen Receptor (AR); Mouse Androgen Receptor (AR) ELISA Kit; AIS; DHTR; HUMARA; KD; NR3C4; SBMA; SMAX1; TFM; Dihydrotestosterone receptor; Nuclear Receptor Subfamily 3; Group C; Member 4; Testicular Feminization; Kennedy Disease; AR rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 899

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AR rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7304901
• NCBI GeneID: 11835
• NCBI Accession #: NP_038504.1
• NCBI GenBank Nucleotide #: NM_013476.4
• UniProt Accession #: P19091
• Molecular Weight: 98,194 Da
• NCBI Official Full Name: androgen receptor
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: Ar
• NCBI Official Synonym Symbols: Tfm; AW320017
• NCBI Protein Information: androgen receptor
• UniProt Protein Name: Androgen receptor
• Protein Family: Allatostatin
• UniProt Gene Name: Ar
• UniProt Synonym Gene Names: Nr3c4

NCBI Description

This gene encodes a nuclear hormone receptor containing zinc finger and DNA-binding domains. The encoded protein is a key regulator of signalling by androgens, a class of steroid hormones involved in male reproductive development. The protein responds to hormone signalling by translocating to the nucleus, forming dimers, and binding to androgen response elements (AREs) in the promoters of target genes, which are subsequently transcriptionally activated. Activity of this protein is negatively regulated by nuclear receptor subfamily 0 group B member 1 (Nr0b1, also known as Dax1). Mutations in this gene result in feminized genitals and infertility in male animals. Loss of function in female animals also causes problems in reproductive development and function. [provided by RefSeq, May 2015]

Uniprot Description

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.