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Typical Testing Data/Standard Curve (for reference only)

Mouse wingless-type MMTV integration site family, member 1 ELISA Kit | WNT1 elisa kit

Mouse Proto-oncogene Wnt-1, WNT1 ELISA Kit

Gene Names
Wnt1; sw; Int-1; Wnt-1; swaying
Reactivity
Mouse
Synonyms
wingless-type MMTV integration site family; member 1; Mouse Proto-oncogene Wnt-1; WNT1 ELISA Kit; Mouse Proto-oncogene Wnt-1 (WNT1) ELISA kit; INT1; Wingless-type MMTV integration site family; member 1 (oncogene INT1) ; WNT1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of human vWF. No significant cross-reactivity or interference between human vWF and analogues was observed.
Sequence Length
370
Samples
Serum, Plasma, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
6.25 ng/ml-400 ng/ml
Sensitivity
1.56 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for WNT1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for vWF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any vWF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for vWF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of vWF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,086 Da
NCBI Official Full Name
proto-oncogene Wnt-1
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 1
NCBI Official Symbol
Wnt1
NCBI Official Synonym Symbols
sw; Int-1; Wnt-1; swaying
NCBI Protein Information
proto-oncogene Wnt-1; proto-oncogene Int-1; proto-oncogene protein Wnt-1; wingless-related MMTV integration site 1
UniProt Protein Name
Proto-oncogene Wnt-1
Protein Family
UniProt Gene Name
Wnt1
UniProt Synonym Gene Names
Int-1; Wnt-1
UniProt Entry Name
WNT1_MOUSE

Uniprot Description

WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted, signal peptide; Oncoprotein

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region

Molecular Function: protein domain specific binding; protein binding; frizzled binding; cytokine activity; receptor binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; multicellular organismal development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; signal transduction; diencephalon development; negative regulation of BMP signaling pathway; BMP signaling pathway; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; positive regulation of cell proliferation; midbrain development; midbrain-hindbrain boundary maturation during brain development; myoblast fusion; positive regulation of Notch signaling pathway; neuron fate determination; organ regeneration; inner ear morphogenesis; Wnt receptor signaling pathway; embryonic axis specification; neuron fate commitment; DNA damage response, signal transduction; myotube differentiation; negative regulation of fat cell differentiation; metencephalon development; organ morphogenesis; negative regulation of cell differentiation; central nervous system morphogenesis; ureteric bud branching; forebrain anterior/posterior pattern formation; midbrain-hindbrain boundary development; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation

Research Articles on WNT1

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Product Notes

The Mouse WNT1 wnt1 (Catalog #AAA908147) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA908147 ELISA Kit recognizes Mouse WNT1. It is sometimes possible for the material contained within the vial of "wingless-type MMTV integration site family, member 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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