Rabbit anti-Human PCDHGA8 Polyclonal Antibody | anti-PCDHGA8 antibody

PCDHGA8, CT (PCDHGA8, KIAA0327, Protocadherin gamma-A8) (PE)

Cat. Num. AAA6331692

• Gene Names: PCDHGA8; PCDH-GAMMA-A8
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHGA8; Polyclonal Antibody; CT (PCDHGA8; KIAA0327; Protocadherin gamma-A8) (PE); Protocadherin gamma-A8; anti-PCDHGA8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PCDHGA8 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCDHGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 788-816 amino acids from the C-terminal region of human PCDHGA8.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PCDHGA8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHGA8 antibody detected the PCDHGA8 protein (arrow).)

Immunohistochemistry (IHC)

(PCDHGA8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHGA8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PCDHGA8 Antibody (C-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PCDHGA8 antibody

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Product Categories/Family for anti-PCDHGA8 antibody

Antibodies; Cadherin

References

Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000). Wu, Q., et al. Cell 97(6):779-790(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 14270484
• NCBI GeneID: 9708
• NCBI Accession #: NP_114477.1
• NCBI GenBank Nucleotide #: NM_032088.1
• UniProt Accession #: Q9Y5G5; O15039; A7MCZ4
• Molecular Weight: 101,480 Da
• NCBI Official Full Name: protocadherin gamma-A8 isoform 1
• NCBI Official Synonym Full Names: protocadherin gamma subfamily A, 8
• NCBI Official Symbol: PCDHGA8
• NCBI Official Synonym Symbols: PCDH-GAMMA-A8
• NCBI Protein Information: protocadherin gamma-A8
• UniProt Protein Name: Protocadherin gamma-A8
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHGA8
• UniProt Synonym Gene Names: KIAA0327; PCDH-gamma-A8
• UniProt Entry Name: PCDG8_HUMAN

NCBI Description

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDHGA8: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: homophilic cell adhesion