Myoglobin Recombinant Protein | MB recombinant protein

Myoglobin, Recombinant, Human (MB)

Cat. Num. AAA636050

• Gene Names: MB; PVALB
• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: Myoglobin; Recombinant; Human (MB); MB recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Recombinant Myoglobin.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Inside the protein the iron is coordinated to HIS 93, the "proximal histidineHIS 64, the "distal histidine" also plays an important role in Mb and Hb.)

Testing Data

(Most of the heme is buried within the protein. The carboxyl groups of the heme are exposed to the solvent.)

Related Product Information for MB recombinant protein

Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O(2) by its heme residue, a porphyrin ring:iron ion complex. Since the initial discovery of its structure over 40 years ago, wide-ranging work by many investigators has added importantly to our understanding of its function and regulation. Functionally, myoglobin is well accepted as an O(2)-storage protein in muscle, capable of releasing O(2) during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O(2) concentration when muscle activity increases and to facilitate intracellular O(2) diffusion by providing a parallel path that augments simple diffusion of dissolved O(2). The use of gene targeting and other molecular biological techniques has revealed important new insights into the developmental and environmental regulation of myoglobin and provided additional functions for this hemoprotein such as scavenging nitric oxide and reactive O(2) species.

Recombinant Human MB is a non-glycosylated polypeptide chain having a molecular mass of 11.67kD.

Product Categories/Family for MB recombinant protein

Molecular Biology; MB-Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 44955888
• NCBI GeneID: 4151
• NCBI Accession #: NP_976312.1
• NCBI GenBank Nucleotide #: NM_203378.1
• UniProt Accession #: P02144; Q52H51; Q5THY7
• Molecular Weight: 17,184 Da
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hypoxia; response to hormone stimulus; brown fat cell differentiation; enucleate erythrocyte differentiation