Mouse CD49a Antibody | anti-CD49a antibody

Anti-Hu CD49a Purified

Cat. Num. AAA1802246

• Gene Names: ITGA1; VLA1; CD49a
• Reactivity: Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: CD49a; Anti-Hu CD49a Purified; alpha 1 integrin; VLA-1 alpha; ITGA1; integrin subunit alpha 1; anti-CD49a antibody

• Host: Mouse
• Reactivity: Human, Non-human primates
• Isotype: Mouse IgG1
• Clone Number: TS2/7
• Specificity: The mouse monoclonal antibody TS2/7 recognizes an extracellular epitope of CD49a, the alpha 1 chain of VLA integrin complex, that serves as an adhesion receptor for collagen IV and laminin 1.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD49a antibody: Flow Cytometry (FC/FACS)(QC tested), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml.
• Immunogen: human CTL line
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Flow Cytometry (FC/FACS)

(Separation of HeLa cells stained using anti-CD49a (TS2/7) purified antibody (concentration in sample 0,6ug/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).)

Related Product Information for anti-CD49a antibody

CD49a is the alpha 1 chain of VLA integrin complex (together with CD29, serving as the beta 1 chain), and is expressed on activated T cells, monocytes, NK cells, cultured neuronal cells, melanoma cells, mesenchymal cells (including smooth muscle cells), fibroblasts, hepatocytes, and microvascular endothelium. It binds to collagen IV and laminin 1. It is important for leukocyte migration into tissues. It is upregulated in inflammatory tissues, such as inflammed intestine.

References

MelssenmM, Olson W, Wages NA, Capaldo BJ, Mauldin IS, Mahmutovic A, Hutchison C, Melief CJ, Bullock TN, Engelhard VH, Slingluff CL: Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma. Oncoimmunology. 2018 Aug 6;7(10):e1490855., URL: https://pubmed.ncbi.nlm.nih.gov/30288359/?from_term=melssen+&from_pos=5
Luque A, Gómez M, Puzon W, Takada Y, Sánchez-Madrid F, Cabañas C: Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem. 1996 May 10;271(19):11067-75., URL: https://pubmed.ncbi.nlm.nih.gov/8626649/?from_term=luque+takada&from_pos=2
Canfield SM, Khakoo AY: The nonintegrin laminin binding protein (p67 LBP) is expressed on a subset of activated human T lymphocytes and, together with the integrin very late activation antigen-6, mediates avid cellular adherence to laminin. J Immunol. 1999 Sep 15;163(6):3430-40., URL: https://pubmed.ncbi.nlm.nih.gov/10477615/?from_term=the+nonintegrin+laminin+binding&from_pos=7
Martrus G, Kautz T, Lunemann S, Richert L, Glau L, Salzberger W, Goebels H, Langeneckert A, Hess L, Poch T, Schramm C, Oldhafer KJ, Koch M, Tolosa E, Nashan B, Altfeld M: Proliferative capacity exhibited by human liver-resident CD49a+CD25+ NK cells. PLoS One. 2017 Aug 9;12(8):e0182532., URL: https://pubmed.ncbi.nlm.nih.gov/28792982/?from_term=martrus+kautz&from_pos=1
Thomson-Luque R, Wang C, Tumgia FB, Xu S, Szekeres K, Conway A, Adapa SR, Barnes SJ, Adams JH, Jiang RH: In-depth phenotypic characterization of reticulocyte maturation using mass cytometry. Blood Cells Mol Dis. 2018 Sep;72:22-33., URL: https://pubmed.ncbi.nlm.nih.gov/30007855/?from_term=in-depth+phenotypic+characterization+of+reticulocyte&from_pos=1
Marquardt N, Kekäläinen E, Chen P, Lourda M, Wilson JN, Scharenberg M, Bergman P, Al-Ameri M, Hard J, Mold JE, Ljunggren HG, Michaëlsson J. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells. Nat Commun. 2019 Aug 26;10(1):3841., URL: https://pubmed.ncbi.nlm.nih.gov/31451696/?from_term=marquardt+n+chen+p&from_pos=1

NCBI and Uniprot Product Information

• NCBI GI #: 124056463
• NCBI GeneID: 3672
• UniProt Accession #: P56199; B2RNU0
• Molecular Weight: 130,848 Da
• NCBI Official Full Name: Integrin alpha-1
• NCBI Official Synonym Full Names: integrin, alpha 1
• NCBI Official Symbol: ITGA1
• NCBI Official Synonym Symbols: VLA1; CD49a
• NCBI Protein Information: integrin alpha-1; VLA-1; laminin and collagen receptor; very late activation protein 1; CD49 antigen-like family member A
• UniProt Protein Name: Integrin alpha-1
• UniProt Gene Name: ITGA1
• UniProt Entry Name: ITA1_HUMAN

NCBI Description

This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA1: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: neuron projection; focal adhesion; cell surface; membrane; plasma membrane; acrosome; perikaryon; integrin complex; lipid raft; external side of plasma membrane

Molecular Function: collagen binding; protein binding; metal ion binding; protein phosphatase binding; receptor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; integrin-mediated signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; axon guidance; neutrophil chemotaxis; extracellular matrix organization and biogenesis; activation of MAPK activity; cell-matrix adhesion; vasodilation; negative regulation of cell proliferation; muscle contraction; cellular extravasation; positive regulation of neuron apoptosis; blood coagulation