BCL2L1 Polyclonal Antibody | anti-BCL2L1 antibody

Anti-BCL2L1 Rabbit pAb

Cat. Num. AAA476969

• Gene Names: BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: BCL2L1; Polyclonal Antibody; Anti-BCL2L1 Rabbit pAb; Apoptosis regulator Bcl X; Apoptosis regulator Bcl-X; Apoptosis regulator BclX; B cell lymphoma 2 like; B2CL1_HUMAN; Bcl 2 like 1; Bcl 2 like 1 protein; Bcl X; Bcl xL; BCL XL/S; Bcl xS; Bcl-2-like protein 1; Bcl2 Like 1; Bcl2 like1; Bcl2 related gene; Bcl2-L-1; BCL2L; Bclx; BclXL; BclXs; DKFZp781P2092; PPP1R52; Protein phosphatase 1 regulatory subunit 52; anti-BCL2L1 antibody

• Host: Rabbit; Source: Human
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 170

• Applicable Applications for anti-BCL2L1 antibody: Western Blot (WB)
• Application Notes: WB: 1:300; Other: 1:1000
• Immunogen: Peptide sequence around aa.60~64 (A-D-S-P-A) derived from human BCL-XL.
• Sensitivity: The antibody detects endogenous level of total BCL2L1 protein.
• Sequence Similarities: Belongs to the Bcl-2 family.
• Subunit Structure: Homodimer. Isoform Bcl-X (L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BCL2L11. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with BAD. Interacts (isoform Bcl-X (L)) with SIVA1 (isoform 1); the interaction inhibits the anti-apoptotic activity. Interacts with BECN1 and PGAM5. Interacts (isoform Bcl-X (L)) with BAX (isoform Sigma). Isoform Bcl-X (L) interacts with IKZF3. Interacts with HEBP2. Isoform Bcl-X (L) interacts with BOP/C22orf29. Interacts with P53/TP53 and BBC3; interaction with BBC3 disrupts the interaction with P53/TP53.

Western Blot (WB)

(Western blot detection of BCL2L1 in 3T3, PC12 and Hela cell lysates using BCL2L1 rabbit pAb (dilution 1:300).Predicted band size:30KDa.Observed band size:30KDa.)

Western Blot (WB)

(Western blot detection of BCL2L1 in 3T3, PC12 and Hela cell lysates using BCL2L1 rabbit pAb (1:1000 diluted).Predicted band size:30KDa.Observed band size:30KDa.)

Related Product Information for anti-BCL2L1 antibody

Entrez Summary: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 4502381
• NCBI GeneID: 598
• NCBI Accession #: NP_001182.1
• NCBI GenBank Nucleotide #: NM_138578.1
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 30
• NCBI Official Full Name: bcl-2-like protein 1 isoform Bcl-X(S)
• NCBI Official Synonym Full Names: BCL2 like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Uniprot Description

Bcl-xL: an antiapoptotic member of the Bcl-2 family. Located at the outer mitochondrial membrane and regulates outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are potent inducers of cell apoptosis. Two alternatively spliced isoforms have been reported.

Protein type: Apoptosis; Autophagy; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: centrosome; cytoplasm; mitochondrial outer membrane; mitochondrion

Molecular Function: BH3 domain binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding

Biological Process: apoptotic mitochondrial changes; cytokinesis; mitotic cell cycle checkpoint; negative regulation of apoptosis; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; release of cytochrome c from mitochondria; response to cytokine stimulus; suppression by virus of host apoptosis