Human KLK12 PicoKine ELISA Kit | KLK12 elisa kit

Human KLK12 PicoKine ELISA Kit

Cat. Num. AAA177579

• Gene Names: KLK12; KLKL5; KLK-L5
• Reactivity: Human
• Synonyms: KLK12 PicoKine; Human KLK12 PicoKine ELISA Kit; Kallikrein-12; DKFZp686H1078; Kallikrein like protein 5; Kallikrein related peptidase 12; Kallikrein12; KLK 12; KLK L5; KLK12; KLKL5; MGC42603; kallikrein-related peptidase 12; KLK12 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human KLK12
• Sequence Length: 254

• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human KLK12 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, A18-N248
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human KLK12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1409|3882|6756|1523|4129|6526; Standard deviation|73.26|213.51|405.36|89.85|251.86|443.76; CV(%)|5.2|5.5|6.0|5.9|6.1|6.8
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.035|0.088|0.159|0.328|0.653|1.002|1.752|2.150
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human KLK12 PicoKine ELISA Kit standard curve )

Related Product Information for KLK12 elisa kit

Principle of the assay: Human KLK12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK12 has been precoated onto 96-well plates. Standards (NSO, A18-N248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK12 amount of sample captured in plate.

Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 9665236
• NCBI GeneID: 43849
• NCBI Accession #: NP_062544.1
• NCBI GenBank Nucleotide #: NM_019598.2
• UniProt Accession #: Q9UKR0; Q9UKR1; Q9UKR2
• Molecular Weight: 11,984 Da
• NCBI Official Full Name: kallikrein-12 isoform 1
• NCBI Official Synonym Full Names: kallikrein related peptidase 12
• NCBI Official Symbol: KLK12
• NCBI Official Synonym Symbols: KLKL5; KLK-L5
• NCBI Protein Information: kallikrein-12
• UniProt Protein Name: Kallikrein-12
• Protein Family: Kallikrein
• UniProt Gene Name: KLK12
• UniProt Synonym Gene Names: KLKL5; KLK-L5
• UniProt Entry Name: KLK12_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK12: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.21.-; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space

Molecular Function: peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis