Structure
(Typical Structure of an Antibody)
IgG2b Negative Control Isotype Control
IgG2b Negative Control (FITC)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
IgG2b Negative Control; IgG2b Negative Control (FITC); IgG2b Negative Control isotype control
Host
Mouse
Reactivity
Human
Isotype
IgG2b
Clone Number
3H2413
Specificity
Can be used as a negative control for direct FITC immunofluorescence staining (eg. Flow Cytometry) when using mouse monoclonal antibodies to human cell surface antigens of isotype IgG2b. This enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Conjugated to fluorescein isothiocyanate (FITC).
Applicable Applications for IgG2b Negative Control isotype control
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: Use 10ul of the optimal working dilution to label 10e6 cells in 100ul. Suitable for whole blood, Ficoll-separated preparations.
Optimal dilutions to be determined by researcher.
Dilution: Flow Cytometry: Use 10ul of the optimal working dilution to label 10e6 cells in 100ul. Suitable for whole blood, Ficoll-separated preparations.
Optimal dilutions to be determined by researcher.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Structure
(Typical Structure of an Antibody)
Structure
(Typical Structure of an Antibody)
Related Product Information for IgG2b Negative Control isotype control
Isotype controls are used to discriminate non-specific binding of mouse IgG2b primary monoclonal antibodies to human cells due to Fc recepter binding or other protein-protein interactions.
Product Categories/Family for IgG2b Negative Control isotype control
Similar Products
Product Notes
The IgG2b Negative Control (Catalog #AAA655508) is an Isotype Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgG2b Negative Control (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG2b Negative Control can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: Use 10ul of the optimal working dilution to label 10e6 cells in 100ul. Suitable for whole blood, Ficoll-separated preparations. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the IgG2b Negative Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2b Negative Control, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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